Browsing by Author "Mulugeta Yonas"

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    Analysis of Selected Circulatory Micro RNAs in Breast Cancer Before and After Chemotherapy
    (Addis Ababa Universty, 2018-09) Mulugeta Yonas ; Seifu Daniel
    Breast cancer is the second most common cause of death from cancer in women. There is now increased prevalence of breast cancer among African women. In Ethiopia it is the leading cancer type constituting 33% of all cancer cases in women. It is a heterogeneous type of disease which comprises of many biologically different and pathologically distinct features that lead to different treatment responses. Tests and procedures for diagnosis and prognosis of breast cancer are still limited to invasive procedures and imaging techniques. Based on their differential expression in disease and with their exceptional stability in biological fluids, microRNAs are noticeable candidates to be used as non-invasive diagnostic and prognostic biomarker. The association of change in expression level of microRNAs with clinicopathological parameters may demonstrate their potential in sorting the heterogeneous disease to specific subgroups for effective treatment options. The purpose of this study was to investigate the differential expression of specific circulatory microRNAs in breast cancer patients and study their association with clinicopathological parameters with samples from healthy volunteers serving as control. Changes in the level of microRNA after completing chemotherapy was also assessed and checked for association with serum CA 15-3 values to see the prognostic potential of specific microRNAs. XIII The levels of specific circulatory microRNAs in patient and control samples were analyzed using qRT-PCR. Serum CA 15-3 values from patient samples collected after completion of chemotherapy were measured using solid phase enzyme linked immunosorbent assay. In this study miRNA 21-5p was significantly over expressed in the serum of breast cancer patients when compared with controls Keywords:Breast cancer,heterogeneous
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    Change in Serum Cardiac and Inflammatory Biomarkers Level Following Chemotherapy Treatment among Female Breast Cancer Patients Attending at Tikur Anbessa Specialized Hospital
    (Addis Abeba University, 2019-08) Getie Muluabay; Seifu Daniel; Mulugeta Yonas
    Chemotherapy is long been recognized that a well- established therapeutic approach for several malignancies including breast cancer. But the clinical efficacy of this drug is often limited by its related cardio toxicity. The assessment of multiple cardiac biomarkers can be used in identifying patients at increased risk and adverse outcomes from chemotherapy. This approach has significant importance to predict which patients will have adverse cardiac effects from chemotherapy as well as treat damage at an earlier stage, preserve patient health, and improve quality of life. Objective: The aim of the present study was to evaluate changes in cardiac and inflammatory biomarkers level following chemotherapy treatment among female breast cancer patients attending at Tikur Anbessa specialized hospital. Materials and methods: Hospital based retrospective study was conducted in 40 female breast cancer patients at TASH. Elecsys 2010 Troponin-T immunoassay analyzer ( to measure serum cTnT level ) , Roch/hitachi cobas c 502 analyzer ( to measure serum CKMB and CRP levels) ) wereand R and D systemic luminex performance assay( to measure serum IL-6 and TNF- used in 40 paired breast cancer patient samples retrieved from a repository specimen of an ongoing thematic research project. Data were analyzed using SPSS software version 23 package and a p value < 0.05 was considered statistically significant. Results: In the female breast cancer patients receiving chemotherapy treatment, the mean serum levels were significantly increased than their respective baselinecTnT, CKMB, CRP and TNF- values. No significance difference was found in the level of IL-6 between the base line and during chemotherapy treatment. 20%, 15%, 14% of the breast cancer patients had elevated cTnT, CK-MB and CRP levels after the three cycles of chemotherapy treatment respectively and 15% of patients had a baseline elevated levels of CRP compared with their respective cutoff value. Conclusion: Cardiac biomarkers are significantly increased up on chemotherapy treatment Key words: chemotherapy, cardio toxicity, cardiac biomarkers, inflammatory markers