Analysis of Selected Circulatory Micro RNAs in Breast Cancer Before and After Chemotherapy
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Date
2018-09
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Addis Ababa Universty
Abstract
Breast cancer is the second most common cause of death from cancer in women. There is now increased prevalence of breast cancer among African women. In Ethiopia it is the leading cancer type constituting 33% of all cancer cases in women. It is a heterogeneous type of disease which comprises of many biologically different and pathologically distinct features that lead to different treatment responses. Tests and procedures for diagnosis and prognosis of breast cancer are still limited to invasive procedures and imaging techniques. Based on their differential expression in disease and with their exceptional stability in biological fluids, microRNAs are noticeable candidates to be used as non-invasive diagnostic and prognostic biomarker. The association of change in expression level of microRNAs with clinicopathological parameters may demonstrate their potential in sorting the heterogeneous disease to specific subgroups for effective treatment options. The purpose of this study was to investigate the differential expression of specific circulatory microRNAs in breast cancer patients and study their association with clinicopathological parameters with samples from healthy volunteers serving as control. Changes in the level of microRNA after completing chemotherapy was also assessed and checked for association with serum CA 15-3 values to see the prognostic potential of specific microRNAs. XIII The levels of specific circulatory microRNAs in patient and control samples were analyzed using qRT-PCR. Serum CA 15-3 values from patient samples collected after completion of chemotherapy were measured using solid phase enzyme linked immunosorbent assay. In this study miRNA 21-5p was significantly over expressed in the serum of breast cancer patients when compared with controls
Keywords:Breast cancer,heterogeneous
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Keywords
Breast cancer,heterogeneous