Browsing by Author "Lulu, Mesfin"

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    Detection of Plasnodiun Falciparun Sporozoites in Naturally Infected Anopheline Species Using Fluorescein Labelled Dna Probe and Pcr Procedures.
    (Addis Ababa University, 1994-06) Lulu, Mesfin; Miorner, Hakan (PhD)
    A dot blot assay to detect P. faicipaTum sporozoi tes from naturally infected An. gambiae s.l was developed. Indoor resting blood fed female An.gambiae s.l were collected from Metehara, Zeway and Arbaminch. These mosquito specimens were assayed for P.faicipaTum sporozoites using the hybridization. method with a fluorescein labelled PFRI oligomer and the Polymerase Chain Reaction (PCR). A total of 198 field collected samples were assayed. P. faicipaTum sporozoi tes were detected from 4 samples; 2 from Zeway and the remaining 2, each from Metehara and Arbaminch by both hybridization and , .... peR methods. Background signal in the hybridization ass~y, generated from blood fed mosquitoes was cleared by keeping mosquito specimens in cages for 48 hrs after collection, so that the majority, if not all of the blood meal was digested before killing and preservation. Results of this study indicate that this method could be used for large scale epidemiological studies.