Detection of Plasnodiun Falciparun Sporozoites in Naturally Infected Anopheline Species Using Fluorescein Labelled Dna Probe and Pcr Procedures.
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Date
1994-06
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Addis Ababa University
Abstract
A dot blot assay to detect P. faicipaTum sporozoi tes
from naturally infected An. gambiae s.l was developed. Indoor
resting blood fed female An.gambiae s.l were collected from
Metehara, Zeway and Arbaminch. These mosquito specimens were
assayed for P.faicipaTum sporozoites using the hybridization.
method with a fluorescein labelled PFRI oligomer and the
Polymerase Chain Reaction (PCR). A total of 198 field
collected samples were assayed. P. faicipaTum sporozoi tes were
detected from 4 samples; 2 from Zeway and the remaining 2,
each from Metehara and Arbaminch by both hybridization and
, ....
peR methods. Background signal in the hybridization ass~y,
generated from blood fed mosquitoes was cleared by keeping
mosquito specimens in cages for 48 hrs after collection, so
that the majority, if not all of the blood meal was digested
before killing and preservation. Results of this study
indicate that this method could be used for large scale
epidemiological studies.
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Biology