Browsing by Author "Lijalem, Teklebrihan"

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    Micropropagation of violet tree (Securidaca longepedunculata, Fresen.), an Endangered Ethiopian medicinal plant from Shoot tip
    (Addis Ababa University, 2014-07) Lijalem, Teklebrihan; Feyissa, Tileye (PhD)
    Securidaca longipedunculata Fresen, locally called Etse-manahi, is semi-deciduous shrub or small tree that belonging to the family Polygalaceae and found in rainfall and equatorial humid areas of Africa. It is an indigenous medicinal plant in Africa and has important place in the traditional medicine as well as modern medicine. This plant becomes endangered because of high seed dormancy, low germination rate, over exploitation and long growth period. Therefore, micropropagation method is important to address these problems. Thus, the objective of this study is to develop micropropagation protocol for S. longipedunculata from shoot tip explants. Seeds were de-coated and disinfected using 3%, 5%, 10% and 15% (v/v) Clorox and cultured on growth regulators-free solid MS medium. Shoot tips were initiated using various concentrations of 6-Benzyl Amino Purine (BAP) in combination with Thidiazuron. MS medium containing different concentrations of BAP in combination with IBA; TDZ in combination with IBA or NAA were used for shoot multiplication. Shoots were cultured on full and half strength MS medium containing IAA, IBA or NAA for root induction. The highest percentage of shoot initiation (87%) was obtained on MS medium containing 1.0 mg/l BAP. Maximum number of shoots (8.5 ± 0.69) was achieved on MS multiplication medium containing 1.5 mg/l BAP in combination with 0.1 mg/l IBA. Shoots gave highest number of roots (3.73 ± 0.69) on full strength MS rooting medium containing 2.0 mg/l IAA. Among 102 plantlets transferred to greenhouse, 61 plantlets survived on the pot containing soil, sand and compost in a ratio of 2:1:1 respectively. Key words/phrases: ex vitro; in vitro; Micropropagation; plant growth regulator, Securidaca longipedunculata, shoot initiation; shoot tip culture