Browsing by Author "Kaleab Zerom"

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    Isolation and Characterization of Mycobacterial Species Causing Tuberculosis Lesions in Slaughtered Camels in Eastern Ethiopia
    (Addis Ababa University, 2011-06) Kaleab Zerom; Tesfaye Sisay; Gobena Ameni; Gezahaegne Mamo
    A cross sectional study was conducted on 293 apparently healthy camels slaughtered at Jigjiga. Harar, Aweday and Dire Dawa abattoirs between December 2010 and April 20 11, The objective of the study was to isolate and characterize Mycobacterial species causing tuberculous lesions III slaughtered camels through postmortem examination. mycobacteria logical culturing. and multiplex polymerase chain reaction (PCR). A prevalence or 12.3% (36 /293) was recorded on the basis of postmortem examination and the di afference was statistically significant in females (X'=7.474. 1'=0.006) and in poor body condition (X'~ 7.323, 1'=0.025) camels when compared to their counter parts. About 79.5% of tuberculous lesions were found in lungs and associated lymph nodes in thoracic cavity and 15.9% in the lymph nodes of the head indicating inhalation as principal route of infection .Of the 36 camels with gross suspicious TB lesion organs and tissues cultured mica bacterial growth was observed in 66.7% (24 /3 6). Genotyping of the 17 isolates using multiplex polymerase chain reaction identified 88.2% (15/ 17) of them as member of mycobacteria other than Mycobacterium liber calliopsis complex and only once as member of M)lcobaCleriul1l ,tuberculosis complex. The relatively higher isolate ion of nontuberculous mycobacteria from most of camel 1'B lesions in this study could indicate their pathogenic or opportunistic role in the pathology or camel 1'13 signifying the need to further examine these species as cause of came l 'I'll so as to c1kctively elucidate its pathology. and enhance the effort in the control 01' the disease. Key words: Camels, Genotyping, Mycobacterium tuberculosis complex. Nontuberculous mycobacteria, Prevalence, Tuberculosis