Isolation and Characterization of Mycobacterial Species Causing Tuberculosis Lesions in Slaughtered Camels in Eastern Ethiopia
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Date
2011-06
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Addis Ababa University
Abstract
A cross sectional study was conducted on 293 apparently healthy camels slaughtered at
Jigjiga. Harar, Aweday and Dire Dawa abattoirs between December 2010 and April 20 11,
The objective of the study was to isolate and characterize Mycobacterial species causing
tuberculous lesions III slaughtered camels through postmortem examination.
mycobacteria logical culturing. and multiplex polymerase chain reaction (PCR). A
prevalence or 12.3% (36 /293) was recorded on the basis of postmortem examination and
the di afference was statistically significant in females (X'=7.474. 1'=0.006) and in poor body
condition (X'~ 7.323, 1'=0.025) camels when compared to their counter parts. About 79.5%
of tuberculous lesions were found in lungs and associated lymph nodes in thoracic cavity
and 15.9% in the lymph nodes of the head indicating inhalation as principal route of
infection .Of the 36 camels with gross suspicious TB lesion organs and tissues cultured
mica bacterial growth was observed in 66.7% (24 /3 6). Genotyping of the 17 isolates using
multiplex polymerase chain reaction identified 88.2% (15/ 17) of them as member of
mycobacteria other than Mycobacterium liber calliopsis complex and only once as member of
M)lcobaCleriul1l ,tuberculosis complex. The relatively higher isolate ion of nontuberculous
mycobacteria from most of camel 1'B lesions in this study could indicate their pathogenic
or opportunistic role in the pathology or camel 1'13 signifying the need to further examine
these species as cause of came l 'I'll so as to c1kctively elucidate its pathology. and enhance
the effort in the control 01' the disease.
Key words: Camels, Genotyping, Mycobacterium tuberculosis complex. Nontuberculous
mycobacteria, Prevalence, Tuberculosis
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Keywords
Camels, Genotyping, Mycobacterium tuberculosis complex. Nontuberculous mycobacteria, Prevalence, Tuberculosis