Browsing by Author "Girma,Minilu"
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Item A comparative study of the leaf and root extracts of Stephania abyssinica (Dillon & A. Rich) Walp on wound healing activity in mice(Addis Ababa University, 2023-07) Girma,Minilu; Petros,Zelalem (PhD); Tamirat,Dagnachew(MD)The roots and leaves of Stephania abyssinica are traditionally used to treat wounds in several regions of Ethiopia. The pharmacological screening for wound-healing activity of the plant was done for the crude extract and solvent fractions of the root extract. But there have been no pharmacological studies done on the wound-healing effect of the leaf extract. In this study, the wound-healing effects of both the 80% methanol extract of the leaves and the roots of S. abyssinica were evaluated using the excision wound model, and the results were compared. Histopathological investigations were also carried out. The antioxidant activity of both the leaf and root extracts was also assessed. In addition, preliminary phytochemical screening tests and quantification of total phenolic, flavonoid, and alkaloid contents were done for both the leaf and root extracts. Both the root and the leaf extracts significantly increased the rate of wound contraction (p < 0.05) and shortened the re-epithelialization period (p < 0.01). The root extract significantly increased the skin‟s tensile strength (p ˂ 0.001). The quantity of secondary metabolites in the root extract, such as total phenolic, flavonoid, and alkaloid contents, was found to be higher than those of the leaf extract, and this concentration difference demonstrated a substantial difference in its wound healing activity.