A comparative study of the leaf and root extracts of Stephania abyssinica (Dillon & A. Rich) Walp on wound healing activity in mice
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Date
2023-07
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Addis Ababa University
Abstract
The roots and leaves of Stephania abyssinica are traditionally used to treat wounds in several
regions of Ethiopia. The pharmacological screening for wound-healing activity of the plant
was done for the crude extract and solvent fractions of the root extract. But there have been
no pharmacological studies done on the wound-healing effect of the leaf extract. In this study,
the wound-healing effects of both the 80% methanol extract of the leaves and the roots of S.
abyssinica were evaluated using the excision wound model, and the results were compared.
Histopathological investigations were also carried out. The antioxidant activity of both the
leaf and root extracts was also assessed. In addition, preliminary phytochemical screening
tests and quantification of total phenolic, flavonoid, and alkaloid contents were done for both
the leaf and root extracts. Both the root and the leaf extracts significantly increased the rate of
wound contraction (p < 0.05) and shortened the re-epithelialization period (p < 0.01). The
root extract significantly increased the skin‟s tensile strength (p ˂ 0.001). The quantity of
secondary metabolites in the root extract, such as total phenolic, flavonoid, and alkaloid
contents, was found to be higher than those of the leaf extract, and this concentration
difference demonstrated a substantial difference in its wound healing activity.
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Keywords
Wound healing, Antioxidant, Stephania abyssinica