Browsing by Author "Gelan, Banchiayehu"

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    In vitro Propagation of Dingetegna (Taverniera abyssinica A. Rich)
    (Addis Ababa University, 2015-06) Gelan, Banchiayehu; Feyissa, Tileye (PhD)
    Taverniera abyssinica A. Rich, commonly known as ‘Dingetegna’ in Amharic in Ethiopia,belongs to family Fabaceae. This species has been threatened as it is used as a major traditional medicine for stomachache, headache and fever in many parts of Ethiopia. In vitro germination and propagation of T. abyssinica has immense value in rapid production and conservation of this species. Therefore, the objective of this study is to develop micropropagation protocol for T. abyssinica using seed as explant. Plant growth regulators (PGRs)-free Murashige and Skoog (MS) medium was used to initiate the growth of shoots from seeds. Shoots were multiplied on the same medium containing different concentrations and combinations of 6-Benzyl amino purine (BAP) in combination with gibberrelic acid (GA3). The rooting experiment was done using half and full strength MS medium supplemented with 2g/l activated charcoal (AC) and 0.4mg/l IBA and NAA. Among the various concentrations of BAP (0.1, 0.2, 0.3, 0.4, and 0.5 mg/l) in combination with GA3 (0.5 or 1 mg/l) used, highest rate of shoot multiplication (5.93±4.08) was obtained on MS medium containing 0.2 mg/l BAP combined with 1.0 mg/l GA3. Moreover, shoots cultured on PGRs- free medium exhibited the highest mean shoot length (3.89±1.33). In vitro developed shoots were rooted best (2.30±1.39) in half strength MS medium supplemented with 2g/l AC and 0.4 mg/l IBA in combination with 0.4 mg/l NAA. Upon transfer to glasshouse, 83.33% of plants survived after six weeks of acclimatization. Key Words: Activated charcoal, Dingetegna, Medicinal plant, Plant growth regulators, Shootmultiplication