Browsing by Author "Gebremedhin, Mulugeta"

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    Bacterial Profiles and Antibiotic Sensitivity Pattern of Isolates from Medical Devices and Inanimate Surfaces from ICU Room at Yekatit 12 Hospital Medical College, Addis Ababa, Ethiopia.
    (Addis Abeba University, 2021-08) Gebremedhin, Mulugeta; Dr. Tesfaye, Abreham(MSc, PhD); Hailu, Melese(BSc, MSc, PhD Candidate)
    Background: Infections acquired in hospitals are a major public health concern around the world, especially when caused by multidrug-resistant (MDR) microorganisms. Bacteria, including MDR isolates, were found in critical care units near the patient or nearby environment (inanimate surfaces and equipment). Microorganisms may be transmitted indirectly via inanimate surfaces and equipment, which could play a substantial role in ICUrelated infections. Objective: To assess bacterial profile and antibiotic sensitivity pattern of isolates from medical devices and inanimate surfaces of ICU room at Yekatit 12 Hospital Medical College, Addis Ababa, Ethiopia. Methods: A hospital-based cross-sectional study was conducted from April to June 2021 in Yekatit 12 Hospital Medical College in Addis Ababa. Swabs were collected twice in 30 days from medical devices and inanimate surfaces of ICU room. A total of 136 samples were collected by swabbing hospital surfaces, and the bacterial isolates were identified using Gram's staining, standard culture, and biochemical tests. Besides, the respective antimicrobial sensitivity tests were checked for antibiotic susceptibility using the Kirby–Bauer disk diffusion. SPSS Version 25 was used to analyzed and present the results in terms of percentage and table. Result Bacterial growth was found in 45 (33.1%) of the 136 samples tested. A total of 56 bacterial pathogens were recovered from all specimens processed during the study. Grampositive bacteria accounted for 16 (28.6%), while Gram-negative bacteria accounted for 40 (71.4%). Among the Gram-positive isolates, Coagulase-negative Staphylococci 13(23.2%) were frequently isolated followed by Bacillus species 2(3.6%). Klebsiella species 21(37.5%) were most isolated of the Gram-negative rods. The range of resistance for Gram-positive and Gram-negative bacteria were from 87.5% – 100% and 30% – 100% respectively. From the study participants, 32(82.1%) clean surfaces of medical equipment and inanimate, and 33 (84.6%) cleaned the reusable equipment before storage or used on next patient. Conclusion: In the ICU, the rate of bacterial contamination found is high and mostly resistance to different types of anti-microbial drugs were prescribed. The infection control and prevention unit in the hospital is better to adopt observation on a regular basis.