Browsing by Author "Daniel Abebe"

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    Isolation and Identification of Corynebacterium Pseudotuberculosis from Superficial Lymphnodes of Sheep and Goat at Organic Export Abbatoir, Modjo Ethiopia
    (2012-06) Daniel Abebe; Tesfaye Sisay
    Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), a disease characterized by the formation of suppurative abscesses, particularly in superficial and internal lymph nodes, and in internal organs in small ruminants. The present study was conducted from November to April 2011/ 2012 only on the superficial lymph nodes and carcass of small ruminants. Out of 768 small ruminants slaughtered at Organic slaughterhouse during the study period, 82 (68 in goats and 14 in sheep) were found to have gross evidence of abscess or caseous lymphadenitis. The typical gross lesion is a discrete abscess distended by thick and often dry, greenish yellow or white, purulent exudates. The most frequent sites of abscesses in goats occurred in the prescapular, prefemoral and poplitial lymph node and carcass 34(5.54%), 24(3.91%), 2(0.33%) and 8(1.30%) respectively. In sheep 7(4.54%) in the prescapular , 5(3.25%) in prefemoral and 0% in poplitial lymph node and 2(1.23%) abscess on carcass were found. The study indicated that in both species young animals are more in number than adult, however, caseous lymphadenitis was found to be higher in adult than young animals in both species. There was statistically significant difference in the occurrence of CLA between age groups (P<0.05). Out of 68 bacterial cultures from goats 18 and 50, pure and mixed colonies, respectively, were obtained; while out of 14 bacterial cultures from sheep 4 and 10, pure and mixed colonies, respectively, were obtained. Corynebacterium pseudotubrculosis isolates were obtained from 59 out of 82 collected abscessed lymph nodes and carcass. Bacterial colonies were identified on the basis of morphological characteristics, primary and secondary biochemical tests, i.e. catalase, urease, trehalose, xylose, maltose, and glucose fermentation tests and reverse CAMP test (antagonistic haemolysis between C. pseudotuberculosis and Staphylococcus aureus). Isolates positive for catalase, urease, maltose, and glucose, but with inhibiting β- haemolysin of S. aureus by pospholipase D of C. pseudotuberculosis and negative for trehalose, and xylose were considered as C. pseudotuberculosis. The susceptibility pattern of C. pseudotuberculosis to antimicrobial agents varied among isolates. Out of 59 isolates of C. pseudotuberculosis isolated from lesions of caseous lymphadenitis in goats and sheep were susceptible to the antibiotics norfloxacin, tetracycline, doxycyline HCl and kanamycine.
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    The Relevance of Style and Structure in the Creation of Social Interaction in Passion’s Treasure
    (Addis Ababa University, 2012-05) Daniel Abebe ; Pradeep Sharma
    The aim of this research paper was to investigate the relevance of style and structure in the creation of social interaction in Passion’s Treasure. In doing this, the study employed qualitative research method specifically case study. To proceed the process, the study uses five chapters with various sub topics The first chapter maintains how the study was going to be studied. This means it gives relevant highlight from where the study began and stopped. The second chapter regulates the review of theoretical aspects. To formulate effective systems, various approaches should be seen deeply and thoroughly. To enhance persuasive findings, new approaches were expected to regulate the analysis. Therefore deviation through contractual sequence, repetition through characterization and parallelism through plot were taken to facilitate the coming chapter. The third chapter entrusts or entrenches the analysis of the novel Passion’s Treasure using the framework that was designed in chapter two. The fourth one made intensive discussion about what we had said previously. That is brave discussion about the outcomes of style and structure to create social interaction. The last chapter concludes the whole study and findings at once with the structure of the novel Passion’s Treasure in the form of chart.