Browsing by Author "Admasu Atnafseged"

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    Determination of Total Gossypolin Cottonseeds and Cottonseed Press Cakes
    (Addis Ababa Universty, 1982-07) Admasu Atnafseged; Chandravanshi B.S. (PhD)
    The reaction of gossypol vuth 3~anuno-l~propauol and iron (III) in acidic medium produces a green colored complex soluble in isopropyl alcohol-fcexa.no mixture (3:2), having a characteristic absorption maximum at 6"o ran, The co-ored reaction product, is sufficiently stable and obeys Beer’s lav: in the concentration range 5 -84 ppn of gossypol, Tlie effective molar absorptivity in terms of goegypol is found to he -1 -1 64f% 1. mole . cm. vdth a photometric sensitivity of 0.08;jg of y gossypol,per an. On tlie basis of sensitive color reaction, a new method, has been developed for the determination'.of gcsaynol, The , effect of several experimental variables on the determination of gossypol have been studied. The stoichiometric composition of the green colored goseypol^aminoprcpanol-iron (III) complex has been determined and was found to be 1:2 (gossypol. to iron) The precision of the method has leon evaluated in terms ofstandard deviation and the relative standard deviation vjarsfound tobe + o.71%.The validity of the method was established by the reccwery of standard gosaypol added to the cottonseed samples, The proposed motljod has been applied for analysis of total gossypol in several varieties of cottonseed grown under different conditions in Ktliiopia and tho cottonseed press cakes available in. the market. The goravrol content of the samples were detorrdned after extraction of total gossypol from tlie cottonseed and cottonseed press cakes by 3~oriino“ l~propanol in dimethylforrtvoride. The results obtained by the proposed method liavc leon compared. with the results obtained by standard A.O.O.S. method. Tho tvjc results were found to be almost identical.