Determination of Total Gossypolin Cottonseeds and Cottonseed Press Cakes
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Date
1982-07
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Addis Ababa Universty
Abstract
The reaction of gossypol vuth 3~anuno-l~propauol and iron (III)
in acidic medium produces a green colored complex soluble in isopropyl
alcohol-fcexa.no mixture (3:2), having a characteristic absorption
maximum at 6"o ran, The co-ored reaction product, is sufficiently stable
and obeys Beer’s lav: in the concentration range 5 -84 ppn of gossypol,
Tlie effective molar absorptivity in terms of goegypol is found to he
-1 -1
64f% 1. mole . cm. vdth a photometric sensitivity of 0.08;jg of
y
gossypol,per an. On tlie basis of sensitive color reaction, a new
method, has been developed for the determination'.of gcsaynol, The ,
effect of several experimental variables on the determination of
gossypol have been studied. The stoichiometric composition of the
green colored goseypol^aminoprcpanol-iron (III) complex has been
determined and was found to be 1:2 (gossypol. to iron) The precision of the method has leon evaluated in terms ofstandard deviation and the relative standard deviation vjarsfound tobe + o.71%.The validity of the method was established by the reccwery of
standard gosaypol added to the cottonseed samples,
The proposed motljod has been applied for analysis of total
gossypol in several varieties of cottonseed grown under different
conditions in Ktliiopia and tho cottonseed press cakes available in.
the market. The goravrol content of the samples were detorrdned
after extraction of total gossypol from tlie cottonseed and cottonseed
press cakes by 3~oriino“ l~propanol in dimethylforrtvoride. The results
obtained by the proposed method liavc leon compared. with the results
obtained by standard A.O.O.S. method. Tho tvjc results were found to
be almost identical.
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Keywords
3~anuno-l~propauol and iron (III)