Investigation of Respiratory Viruses in Camels Slaughtered at Addis Ababa Akaki Abattoir
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Date
2011-06
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Addis Ababa University
Abstract
This study was conducted from September 20 10- June 20 11 at Debre Zeit National Veterinary
Institute (NVI) with an attempt to isolate and characterize respiratory viruses from pneumonic
lungs of camels slaughtered at Addis Ababa Akaki abattoir. Accordingly, pneumonic camel lung
tissues were collected and transported in ice box to NVI virology laboratory and temporarily
stored at -85°C and then processed and inoculated on confluent vero cells mono layers, incubated
at 37°C and followed for the development of CPE for 4-15 days. All CPEs observed developed
between 4-14 days. Thirty five (35) pneumonic camel lungs were cultured among which 27 of
them were CPE positive characterized majorly by initial rounding of vero cells, elongation,
syncytia formation, swelling and rounding, floating and sloughing of cells. Some of the
supernatant suspensions of those samples exhibiting CPEs were taken and extracted both for
DNA and RNA viral genomes. cDNAs were synthesized from RNA extracted samples. The
DNAs and cDNAs were used to test both DNA and RNA viruses, respectively using universal
(DOP- PCR) and conventional PCR techniques. Respiratory syncytial virus, Adenovirus, Pest des
polities ruminants virus, Parainfluenza I virus, Parainfluenza 2 virus and Parainfluenza 3 virus
were tested. All six samples tested were negative for PPRV and Parainfluenza virusesl-3. But
nine of ten samples tested were positive for Respiratory syncytial virus and two of six samples
tested were positive for Adenovirus. Finally, further investigative, epidemiological and molecular
characterization studies were forwarded .
Key words: Abattoir, Camel, Polymerase chain reaction, Respiratory viruses, Virus isolation