In Vitro Propagation of Anchote (Coccinia Abyssinica(Lam.) Cogn) From Shoot Tip Explants
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Date
2018-09-09
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Addis Ababa University
Abstract
Among the major root and tuber crops, anchote (Coccinia abyssinica (Lam.) Cogn) is a potencial tuber crop mostly cultivated in western and southern parts of Ethiopia. It serves as a food, social-cultural, medicin and economic plant for the farming communities. The plant is commonly propagated by seeds and as pollination may occur, it is difficult to obtain true-to-type plants. Therefore, in vitro propagation was used to overcome this problem. This study was conducted to determine the effect of: seed coat on germination, different concentration of NaOCl and varying exposure time on explant sterilization, different concentration of BAP on shoot initiation, different concentration of BAP alone and in combination with NAA on shoot multiplication, different types and concentration of rooting hormones on root induction, and to investigate the percentage of survival after acclimatization. The decoated seeds resulted in higher germination (67%) than coated seeds (43%) after three weeks of sowing. Ten percent NaOCl (v/v) resulted in 60% sterilized explants for 15 min exposure. The highest mean shoot number per explant (2.33±0.88) was obtained on MS medium containing 1.0 mg/l BAP during shoot culture initiation. During multiplication, the maximum shoot numbers per explant (4.40±1.73 and 4.40±1.30) were obtained on MS medium supplemented with 1.0 mg/l BAP in combination with 0.10 mg/l NAA and 1.50 mg/l BAP in combination with 0.10 mg/l NAA respectively. The maximum root numbers (12.27±3.58) and the maximum root length (5.98±1.13 cm) were obtained on the half strength MS medium containing 0.25 mg/l IAA. The percentage of survival after acclimatization was 78%. This protocol can be used to produce large number of disease free clean planting materials of anchote within short period of time. So that, this study could be used for agricultural, medicinan, industrial and commercial production of anchote in good quality and quantity.
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Coccinia Abyssinica (Lam.) Cogn, In Vitro Propagation, MS Medium, and Plant Growth Regulator