Characterization of antagonistic Trichoderma species against Coffee Wilt Disease (Fusarium xylarioides)
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Date
2010-12
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Addis Ababa University
Abstract
In vitro antagonistic activity of Trichoderma isolates against coffee wilt disease (CWD), Fusarium
xylarioides (Gibberella xylarioides) was studied. Out of 32 soil samples and 7 coffee tree parts
collected from 6 different coffee growing woredas of Jimma Zone, 7 potential/effective
Trichoderma isolates were isolated and characterized out of 74 fungal cultures. Trichoderma
isolates were characterized based on spore morphology and vegetative growth characterizations on
different growth media, temperature and pH ranges. The most suitable and maximum growth of
Trichoderma isolates was obsereved at pH 4.5 and 5.5 in all of the isolates. In vitro evaluation of
dual culture test was done for each of the seven Trichoderma isolates against F. xylarioides. It was
observed that all of the seven Trichoderma isolates have shown 63.33 to 70.95 percent of mycelial
growth inhibition of F. xylarioides after 6 days of incubation at 250C on potato dextrose agar
medium. Trichoderma isolate AUT4 was the most effective biocontrol agent which exhibited the
maximum inhibition of 70.95%. Whereas, the least percent of mycelial growth inhibition 63.33%
was obtained by Trichoderma isolate AUT3. In addition to the above, the seven isolates of
Trichoderma were then tested for their production of volatile antifungal compounds against F.
xylarioides. They produced volatile compounds which inhibited the mycelial gorwth of the test
pathogen from 66 to 80.60%. Trichoderma isolate AUT1 produced the most effective volatile
compounds which inhibited growth by 80.60%. Therefore, the application of in vitro evaluations of
seven Trichoderma isolates has proved to be highly potential/effective in the control of coffee wilt
diseases (Fusarium xylarioides).
Key words: Biological Control agents, cofffee wilt disease, Fusarium xylarioides,
Trichoderma isolates.
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Keywords
Biological Control agents, cofffee wilt disease, Fusarium xylarioides, Trichoderma isolates