ISOLATION AND IDENTIFICATION OF Corynebacterium pseudotuberculosis FROM SUPERFICIAL LYMPHNODES OF SHEEP AND GOAT AT ORGANIC EXPORT ABBATOIR, MODJO ETHIOPIA
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2012-06
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Abstract
Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), a disease characterized by the formation of suppurative abscesses, particularly in superficial and internal lymph nodes, and in internal organs in small ruminants. The present study was conducted from November to April 2011/ 2012 only on the superficial lymph nodes and carcass of small ruminants. Out of 768 small ruminants slaughtered at Organic slaughterhouse during the study period, 82 (68 in goats and 14 in sheep) were found to have gross evidence of abscess or caseous lymphadenitis. The typical gross lesion is a discrete abscess distended by thick and often dry, greenish yellow or white, purulent exudates. The most frequent sites of abscesses in goats occurred in the prescapular, prefemoral and poplitial lymph node and carcass 34(5.54%), 24(3.91%), 2(0.33%) and 8(1.30%) respectively. In sheep 7(4.54%) in the prescapular , 5(3.25%) in prefemoral and 0% in poplitial lymph node and 2(1.23%) abscess on carcass were found. The study indicated that in both species young animals are more in number than adult, however, caseous lymphadenitis was found to be higher in adult than young animals in both species. There was statistically significant difference in the occurrence of CLA between age groups (P<0.05). Out of 68 bacterial cultures from goats 18 and 50, pure and mixed colonies, respectively, were obtained; while out of 14 bacterial cultures from sheep 4 and 10, pure and mixed colonies, respectively, were obtained. Corynebacterium pseudotubrculosis isolates were obtained from 59 out of 82 collected abscessed lymph nodes and carcass. Bacterial colonies were identified on the basis of morphological characteristics, primary and secondary biochemical tests, i.e. catalase, urease, trehalose, xylose, maltose, and glucose fermentation tests and reverse CAMP test (antagonistic haemolysis between C. pseudotuberculosis and Staphylococcus aureus). Isolates positive for catalase, urease, maltose, and glucose, but with inhibiting β- haemolysin of S. aureus by pospholipase D of C. pseudotuberculosis and negative for trehalose, and xylose were considered as C. pseudotuberculosis. The susceptibility pattern of C. pseudotuberculosis to antimicrobial agents varied among isolates. Out of 59 isolates of C. pseudotuberculosis isolated from lesions of caseous lymphadenitis in goats and sheep were susceptible to the antibiotics norfloxacin, tetracycline, doxycyline HCl and kanamycine.
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Antimicrobial agents, Caseous lymphadenitis, Corynebacterium pseudotuberculosis, Lymph nodes