Isolation, Identification, Antimicrobial Profile and Molecular Characterization of Enterohaemorrhagic E. Coli o157:h7 Isolated from Ruminants Slaughtered at Debre zeit Elfora export Abattoir and Addis Ababa Abattoirs Enterprise
No Thumbnail Available
Date
2015-10
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababauniversity
Abstract
A cross sectional study was conducted from December 2014 to June 2015 on apparently
healthy ruminants slaughtered at Debre Zeit ELFORA export abattoir and Addis Ababa
Abattoirs Enterprise in order to determine the prevalence of E. coli with special emphasis
on E. coli O157:H7 and to assess its antimicrobial resistance pattern. The study was also
aimed to molecularly characterize the shiga toxin producing E. coli strains using
multiplex PCR technique. 635 carcass swab samples were collected and processed
according to the standard to isolate and identify the pathogen that lead to an overall
prevalence of 12.9% (E. coli) and 5.5 % (E. coli O157:H7). The prevalence of E. coli
O157:H7 recorded had a statistical difference among animals species and age groups
(P<0.05). However, the result showed that the prevalence of E. coli O157:H7 among
animals’ origin was not found to be statistically significant (P<0.05). The prevalence of
E. coli O157:H7 was higher in the Addis Ababa abattoir enterprise than that of the
privately owned abattoir. Furthermore, E. coli isolates were subjected to the in vitro
antimicrobial sensitivity test and the result indicated the presence of different degrees of
susceptibility and resistance to most of the antibiotics. The degree of susceptibility ranges
from 14.03% up to 85.75% and resistance it is between 5.75% and 48.6%. Out of the 35
E. coli O157:H7 isolates, only 5 (5.7%) of them showed the presence of multidrug
resistance. Analysis of multiplex PCR assay in detecting the virulence genes revealed that
3 out of 20 (15%) were positive in which only one isolate were found to contain both
Stx1 and Stx2 genes. In conclusion, the presence of E. coli O157:H7 in carcasses that
developed resistance can be considered as potential pathogens to the society having the
habit of eating uncooked meats. Therefore, strict measures like practicing proper hygiene
in abattoirs and antibiogram test to select effective drugs should be implemented.
Keywords: Addis Ababa Abattoirs Enterprise, Antimicrobial susceptibility test,
Contamination, E. coli O157:H7, ELFORA export abattoir, PCR, Prevalence, Ruminants
Description
Keywords
Addis Ababa Abattoirs Enterprise, Antimicrobial susceptibility test, Contamination, E. coli O157:H7, ELFORA export abattoir, PCR, Prevalence, Ruminants