Profiles of Physicochemical Characteristics, Antibiotic Residues, Antibiotic Resistance Genes and Bacteria Community Structure of Batu Tannery Wastewater Released to Little Akaki River
| dc.contributor.advisor | Assefa, Fasil (PhD) | |
| dc.contributor.advisor | Feleke, Adey (PhD) | |
| dc.contributor.advisor | G. Love, Nancy (Professor) | |
| dc.contributor.author | Admassu, Tesfaye | |
| dc.date.accessioned | 2021-08-03T09:12:10Z | |
| dc.date.accessioned | 2023-11-18T09:50:19Z | |
| dc.date.available | 2021-08-03T09:12:10Z | |
| dc.date.available | 2023-11-18T09:50:19Z | |
| dc.date.issued | 2020-06-10 | |
| dc.description.abstract | Tanneries produce one of the highest COD wastewaters afterusing large volume of water and different chemicals to produce semi-finished and finished leather products. In Ethiopia, most of the tannery wastewater is discharged directly into water bodies with little or no treatment. These days, different treatment systems are constructed to treat tannery wastewaters that necessitate research to evaluate the efficiency. To this end, a research was conducted on Batu Tannery wastewater treatment plant to evaluate the physicochemical characteristics, bacteria community dynamics, antibiotic residues and antibiotic resistance genes in the different stages of the tannery wastewater treatment plant. The raw tannery wastewater showed variable strength from time to time, andthe highest mean COD (7204+8) mg/l, ammonia-N (190±3.6) mg/l, total nitrogen (308±2)mg/l, and total chromium (22±2.7)mg/lwere observed in April samples. The range of pH remains between 8.0 and 9.0 in raw tannery wastewater and 7.2 and 8.6 in the final effluent. The tannery effluent affected the physicochemical characteristics of the downstream river water of Little Akaki, except temperature and pH. The tannery effluent released to the Little Akaki River caused an increase in the physicochemical values by an average of 3.8%, 9% and 9.5% for COD; 23%, 10.4% and 12.5% for TN; 13.5%, 18.4% and 14.8% forammonia_N; and 31.5%, 28% and 52.8% for total chromium in the November, February, and April samples, respectively. However, only the COD, sulfate and total chromium cause significant (p < 0.05) difference in the downstream river water after tannery effluent was released. 16S rRNA amplicon-based bacterial community analysis from the treatment plant revealed that Firmicutes (48.5%), Bacteroidetes (32.6%) and Proteobacteria (11.5%) were the most represented bacterial phyla across the treatment plant. At the genus level, the dominant genera were Clostridium (15%), Synergistes (5%), Psychrobacter (4%), Acinetobacter (2.5%), Bacteroidetes (1.8%), Anaerovorax (1.3%), Arcobacter (1.3%) and Shewanella (1%). Most of the core bacteria communities in the tannery wastewater were common signatures of gastrointestinal bacteria of ruminants. Some of the core bacteria arepotential pathogens tohuman including the Clostridium, Acinetobacter, Arcobacter and Shewanella. Antibiotic residues and antibiotic resistance genes were detected in the tannery wastewater. The antibiotic residues of tetracycline, oxytetracycline, erythromycin, penicillin G, trimethoprim, sulfadiazine, sulfadoxine, sulfamethoxazole and ciprofloxacin were present in all samples of the tannery wastewater, but penicillin V, amoxicillin, streptomycin, ampicillin and vancomycin were not detected in the same samples. The antibiotic resistance genes tet(A), tet(O), tet(M), erm(B), Sul(I), Sul(II) and Otr(A) were also detected in the tannery wastewater samples. An assessment on the use trend of antibiotics in livestock indicated that antibiotic contamination to tannery wastewater was highly likely to come from the misuse ofantibiotics in livestock disease treatment. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/27555 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Antibiotic Residues | en_US |
| dc.subject | Bacteria Diversity | en_US |
| dc.subject | Illumina Miseq | en_US |
| dc.subject | Livestock | en_US |
| dc.subject | Potential Pathogens | en_US |
| dc.subject | Skin/Hide | en_US |
| dc.subject | Tannery Wastewater | en_US |
| dc.title | Profiles of Physicochemical Characteristics, Antibiotic Residues, Antibiotic Resistance Genes and Bacteria Community Structure of Batu Tannery Wastewater Released to Little Akaki River | en_US |
| dc.type | Thesis | en_US |