Comp Arison of the Activ Ation of Function of T-Cells from Blood and Tissue Lesions of Leprosy Patients
| dc.contributor.advisor | C., Rawleigh (PhD) | |
| dc.contributor.author | Meseret, Yohannes | |
| dc.date.accessioned | 2018-07-17T12:34:38Z | |
| dc.date.accessioned | 2023-11-08T16:34:20Z | |
| dc.date.available | 2018-07-17T12:34:38Z | |
| dc.date.available | 2023-11-08T16:34:20Z | |
| dc.date.issued | 1994-06 | |
| dc.description.abstract | While characterizing the functional properties of T -cells across the leprosy spectrum, significant differences between proliferation ofT-cells isolated from blood and tissue lesions were consistently noted. The differences occurred in more than 80% of cases when comparison was made between blood and skin derived lymphocytes from leprosy patients; in 50-80% cases when comparing lymphocytes isolated from nerves with those from blood of leprosy patients, and > 85 % when comparing lymphocytes derived from blood or skin lesions of patients with other skin inflammatory diseases. The differences were seen throughout the leprosy spectrum. Skin derived lymphocytes from patients with multibacillary (ME) or paucibacillary (PB) leprosy proliferated poorly when compared to lymphocytes isolated from blood and lymphocytes derived from nerves of some PB and most MB patients proliferated poorly when compared to lymphocytes from blood. Stimulation with a strong co-mitogen did not restore responsiveness of T -cells isolated from skin lesions. Cells from different tissues also differed from each other in activation requirements. Analysis of molecules known to differ between T-cell subpopulations and to playa role in T -cell activation indicated some differences in the molecules expressed between blood and tissue lesion derived T-cells. However, the differences observed did not appear to be striking enough to account for the poor responsiveness of tissue lesion derived T-cells. Assays for lymphokine production showed that more IL-2 and IL-4 were produced by T-cell cultured from blood compared to cells from skin, IFN-/, production was found to be made in equal amount by cells from both tissues, The absence of marked differences between blood and lesion derived T-cells, except ill the production of IL-2 and IL-4, indicates that a possible explanation for the difference in proliferation is anergy as anergic cells are not able to produce IL-2 or respond to IL-2 from an external source. Other possibilities which may contribute to the difference are discussed | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/9044 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Biology | en_US |
| dc.title | Comp Arison of the Activ Ation of Function of T-Cells from Blood and Tissue Lesions of Leprosy Patients | en_US |
| dc.type | Thesis | en_US |