Ethanolic Extracts of Warburgia Ugandensis Against Some Test Microorganisms.
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Date
2010-04
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Addis Ababa University
Abstract
Warburgia ugandensis Sprague (Family: Canellaceae) is a tree that is highly utilized in traditional medicine in tropical and warm substropical countries of Africa. Crude ethanol extracts of leaves and heartwood of W. ugandensis were tested for in-vitro antimicrobial activity against six bacterial and a fungi test organisms using the disk diffusion method, agar well diffusion and broth dilution for minimum inhibitory concentration. E. coli was found to be the most susceptible bacterial isolate in the Agar well diffusion and Broth dilution. Meanwhile Shigella boydii was the most resistant bacterial isolate showing MIC value of 10mg/ml for the leaf extract. Bacillus subtilis was the second resistant bacterial isolate giving 0.0mm and 11.0mm inhibition diameter at disk diffusion assay and agar well diffusion, respectively. Streptococcus pneumoniae gave 2.5mg/ml and 5mg/ml MIC value for heartwood and leaf extracts, respectively. The same Minimum Inhibitory Concentration (MIC) value was recorded for B. subtilis. Pseudomonas aeruginosa showed the highest inhibition diameter (16.7mm) in the agar well diffusion assay for the heartwood extract. Staphylococcus aureus and Pseudomonas aeruginosa showed the same MIC value of 2.5mg/ml in leaf and heartwood extract. Candida albicans showed the highest inhibition diameter of 10.0mm and 30.7mm in disk diffusion and agar well diffusion, respectively at the highest test concentration (25mg/ml). In the broth dilution, a MIC value of 1mg/ml concentration of the leaf and heartwood extracts of W. ugandensis inhibited the growth of Candida albicans. It can be concluded that W. ugandensis has the potential as an antimicrobial agent in the future especially against C. albicans and E. coli.
Key words: antimicrobial activity, Warburgia ugandensis, medicinal plants, leaf and heartwood extracts
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Keywords
antimicrobial activity, Warburgia ugandensis, medicinal plants, leaf and heartwood extracts