Profile of Antibody Production Against Schistosoma Mansoni in Mice Immunized With Soluble Adult Worm Antigen
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Date
2000-06
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Addis Ababa University
Abstract
The profile of serum antibody levels against soluble adult worm antigen (SA WA) was
examined in Swiss Albino white mice over a period of 60 days of infection. A group of
mice (n= 15) were immunized with 0.2 mg SA WA through the oral route. Seven days
after immunization, the mice were exposed to 100 cercariae of S. lIlansoni. Another
group (n=15) consisted of un immunized mice that were also exposed to 100 cercariae of
S. lIlansoni. Serum samples were pooled from 4 mice within each group at 0, 7, IS, 30,
45 and 60 days post- infection and the levels of anti-SA W A antibodies determined by
ELISA by using class and sub-class specific monoclonal antibodies. The results revealed
significant difference in the levels of total IgG, and IgG I antibodies in the sera of
immunized mice compared to the unimmunizcd infected mice. In contrast, the IgM
antibody responses were lower in the sera of immunized mice than in the unimmunized
infectedl11ice. Some antibody responses (IgG, IgG I and IgM) began to rise between 15
and 30 days post-infection. Furthermore, IgG2a, IgG3 and IgE antibody responses
positively correlated with the time of the onset of egg deposition. On the other hand, IgA
and IgE were not detected in the immunized and the unimmunizedmice. The profile of
IgG secreting spleen lymphocytes li'otH the two groups of mice showed a similar pattern
of response before the onset of egg deposition (i.e. 45 days). However, the response of
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IgG secreting mesenteric lymphocytes \yas iJ1iiiHtec:' eqrliPi' , in the'tmftllmiiecl lilice, '[h~lli
, ,<", '_' ({ '. , , , " 'l, " <, ,,( , « ,
in the unimmunized infected mice implyillg :tlla,t ~dscin:~t\(i'; !laJ, phlijr,d.il1Y:,lhe's'~litei'ic'
immune apparatus. The single immuniz;ltion~ s~h~~llh~ l;;'~d :ih ilii'; ;t'l:dy; re:sult~d in ar;
increased level of protective antibodies (lgG and Ig;G;I) :ag~)ils;:~\:liiG:!bs::Jjldasi~ ,'manson\
and a decreased level of IgM antibody (Ivhich is,k\101~n:tobe:a~sDd'at~d:\\~ith ;;1~~k1ng of
protective immune response). However, the production of protective antibodies did not
result in a significant reduction in the number of recovered worms. Therefore, in view of
the fact that the relative quantities of protective antibodies are raised following
immunization with SAWA, it will bc necessary to use other parameters such as the
amount and extent of granuloma formation, the viability of eggs, etc., before we conclude
lack of protection by the immunization schedule used in this study.
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Biology