In vitro propagation of Satureja punctata (Benth.) Briq. – A rare aromatic and Medicinal plant from Ethiopia using Shoot tip Explants
No Thumbnail Available
Date
2015-09
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababa University
Abstract
Satureja punctata is an important medicinal plant in Lamiaceae family. This plant is threatened
mainly due to overgrazing, deforestation, over harvesting of the whole plant for medicinal
purpose. This study was aimed to develop an efficient in vitro propagation protocol for S.
punctata using shoot tips. In vitro germinated seedlings were used as mother plant for culture
induction. Shoots excised from thirty-day-old seedlings were cultured on Murashige and Skoog
(MS) medium supplemented with different concentrations of 6-benzyl amino purine (BAP) and
Kinetin (KIN) for shoot induction and multiplication. Half strength MS medium containing
different concentrations of Indole-3- butyric acid (IBA), Indole-3-acetic acid (IAA) and α-
naphthalene acetic acid (NAA) were used for root induction. BAP at 0.5mg/l concentration and
plant growth regulator free medium induced the highest frequency (100%) of shoot induction.
Maximum mean number of shoot per explant (8.25±1.64) and shoot length (2.62±017 cm) were
obtained on medium containing 0.5 mg/l BAP and PGRs free medium respectively. The
combination of BAP with NAA found to be an optimum concentration producing 26.20±2.71
shoots per explant and 2.48±0.13 cm shoot length was obtained from PGRs free medium for
shoot multiplication. MS medium supplemented with 1.0 mg/l IBA gave the highest rooting
percentage (100%) with optimum root number (5.90±0.48) and root length (1.55±0.11cm). Up
on acclimatization, 97.5% survival rate was observed on soil composed of garden soil, sand and
farm yard manure at a ratio of 2:1:1, respectively. The results demonstrated that, this study was
very important for mass propagation and ultimate conservation of this valuable plant.
Key words; Acclimatization; in vitro propagation;, medicinal plant; plant growth regulators;
Satureja punctata
Description
Keywords
; Acclimatization; in vitro propagation;, medicinal plant; plant growth regulators; Satureja punctata