In vitro propagation of Satureja punctata (Benth.) Briq. – A rare aromatic and Medicinal plant from Ethiopia using Shoot tip Explants

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Date

2015-09

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Addis Ababa University

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Satureja punctata is an important medicinal plant in Lamiaceae family. This plant is threatened mainly due to overgrazing, deforestation, over harvesting of the whole plant for medicinal purpose. This study was aimed to develop an efficient in vitro propagation protocol for S. punctata using shoot tips. In vitro germinated seedlings were used as mother plant for culture induction. Shoots excised from thirty-day-old seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyl amino purine (BAP) and Kinetin (KIN) for shoot induction and multiplication. Half strength MS medium containing different concentrations of Indole-3- butyric acid (IBA), Indole-3-acetic acid (IAA) and α- naphthalene acetic acid (NAA) were used for root induction. BAP at 0.5mg/l concentration and plant growth regulator free medium induced the highest frequency (100%) of shoot induction. Maximum mean number of shoot per explant (8.25±1.64) and shoot length (2.62±017 cm) were obtained on medium containing 0.5 mg/l BAP and PGRs free medium respectively. The combination of BAP with NAA found to be an optimum concentration producing 26.20±2.71 shoots per explant and 2.48±0.13 cm shoot length was obtained from PGRs free medium for shoot multiplication. MS medium supplemented with 1.0 mg/l IBA gave the highest rooting percentage (100%) with optimum root number (5.90±0.48) and root length (1.55±0.11cm). Up on acclimatization, 97.5% survival rate was observed on soil composed of garden soil, sand and farm yard manure at a ratio of 2:1:1, respectively. The results demonstrated that, this study was very important for mass propagation and ultimate conservation of this valuable plant. Key words; Acclimatization; in vitro propagation;, medicinal plant; plant growth regulators; Satureja punctata

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; Acclimatization; in vitro propagation;, medicinal plant; plant growth regulators; Satureja punctata

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