In Vitro Propagation of Satureja Punctata (Benth.) Briq. – A Rare Aromatic and Medicinal Plant from Ethiopia using Shoot Tip Explants
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Date
2015-09
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Addis Ababa University
Abstract
Satureja punctata is an important medicinal plant in Lamiaceae family. This plant is threatenedmainly due to overgrazing, deforestation, over harvesting of the whole plant for medicinalpurpose. This study was aimed to develop an efficient in vitro propagation protocol for S.punctata using shoot tips. In vitro germinated seedlings were used as motherplant for cultureinduction. Shoots excised from thirty-day-old seedlings were cultured on Murashige and Skoog(MS) medium supplemented with different concentrations of 6-benzyl amino purine (BAP) andKinetin (KIN) for shoot induction and multiplication. Half strength MS medium containingdifferent concentrations of Indole-3- butyric acid (IBA), Indole-3-acetic acid (IAA) and α-naphthalene acetic acid (NAA) were used for root induction. BAP at 0.5mg/l concentration andplant growth regulator free medium induced the highest frequency (100%) of shoot induction.Maximum mean number of shoot per explant (8.25±1.64) and shoot length (2.62±017 cm) wereobtained on medium containing 0.5 mg/l BAP and PGRs free medium respectively. Thecombination of BAP with NAA found to be an optimum concentration producing 26.20±2.71shoots per explant and 2.48±0.13 cm shoot length was obtained from PGRs free medium forshoot multiplication. MS medium supplemented with 1.0 mg/l IBA gave the highest rootingpercentage (100%) with optimum root number (5.90±0.48) and root length (1.55±0.11cm). Upon acclimatization, 97.5% survival rate was observed on soil composed of garden soil, sand andfarm yard manure at a ratio of 2:1:1, respectively. The results demonstrated that, this study wasvery important for mass propagation and ultimate conservation of this valuable plant.
Key words; Acclimatization; in vitro propagation;, medicinal plant; plant growth regulators;Satureja punctata
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Keywords
; Acclimatization; in vitro propagation;, medicinal plant; plant growth regulators; Satureja punctata