Adaptation of Inactivated Mycoplasma Gallisepticum Vaccine in Ethiopia
No Thumbnail Available
Date
2020-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
However Mycoplasmosis is economically very important disease in poultry farm, but still the disease is undermined in Ethiopia. There was no more study and adapted vaccine on this infectious disease. This experimental study was taken from December 2018 up to May 2020 at National Veterinary Institute, Bishoftu, Ethiopia. The objective of this study was to adapt inactivated Mycoplasma gallisepticum vaccine from National veterinary gene bank, to determine appropriate media which already used by national veterinary institute for production of mycoplasma vaccine of both contagious bovine pleuropnemonia and contagious caprine pleuropnemonia, for growth of Mycoplasma gallisepticum bacteria and to evaluate the adapted inactivated vaccine, safety by follow up of vaccinated chicken for seven days for any clinical sign and death. The media selection was by important three experiments which were optical density, power of hydrogen meter reading and titration. Identification of Mycoplasma gallisepticum vaccine strain was conducted by conventional polymerase chain reaction using species specific primers targeting Mgc2 gene of Mycoplasma gallisepticum at 185bp before using for vaccine production. Polymerase chain reaction confirmed strain was used for the production of oil based formaldehyde inactivated Mycoplasma gallisepticum vaccine. In this study each activity was done according to national veterinary institute standard operation procedure. A total of 60 chickens were used for this experimental study. Chickens were grouped into three groups. All purchased chickens were screened by indirect Enzyme link immune sorbent assay test against mycoplasma gallisepticum anti body. The identity test of mycoplasma gallisepticum vaccine strain was positive at 185bp. The experiment indicates that contagious caprine pleuropnemonia growth media was proper media for growth of Mycoplasma gallisepticum vaccine. All chickens were free from mycoplasma gallisepticum anti body. The vaccine was considered to safe against Mycoplasma gallisepticum vaccine if the number of surviving vaccinated chickens that show no clinical sign and death at the end of the experiment.
Description
Keywords
inactivated vaccine, mycoplasma gallisepticum, National veterinary institute and polymerase chain reaction