Production of Foot-And-Mouth Disease (Serotype: O, A And Sat-2) Polyclonal Antibodies (Igy) By Using Layer Hens
| dc.contributor.advisor | Dr. Shimeles Tesfaye, Dr. Fanos Tadesse | |
| dc.contributor.author | Ephrem, Shimelis | |
| dc.date.accessioned | 2021-03-12T06:48:08Z | |
| dc.date.accessioned | 2023-11-30T12:54:22Z | |
| dc.date.available | 2021-03-12T06:48:08Z | |
| dc.date.available | 2023-11-30T12:54:22Z | |
| dc.date.issued | 2020-09 | |
| dc.description.abstract | An experimental study on FMDV (O, A and SAT-2) polyclonal antibody production trials was conducted from January, 2020 up to August, 2020 G.C. The objective of this study was production of polyclonal antibody from chickens immunized with FMDV. The experimental chickens were grouped in to four where each group consists of eight birds except the control group an inoculated at day 0 and day 14 with quantified dose of serotype O; A, and SAT-2. The immunized chickens were sampled at day 14, 21 and 28 post immunization. Totally 144 sample (72 serum and 72 egg yolk) were pooled by two according to its collection day, serotype and antibody sources in order to make laboratory tests feasible. Non-structural protein detection using ELISA showed the percentage of inhibition (PI) less (< 50%) for all samples detected. At day 14, 21 and 28 post immunization the mean Egg yolk IgY were 10, 8.6 and 13.46 respectively. The result of VNT titer indicated that samples with less Percentage of inhibition, neutralized the homologous FMDV serotypes (Titer TCID log10 > 0.3). On the other hand, the mean antibody titer at day 14, 21 and 28 were 1.83, 2.1 and 2.07 respectively. Even though antibody percentage of inhibition and antibody titer were low, there was a linear relationship between the egg yolk IgY and serum antibody titer. The difference between egg yolk IgY and serum antibody were only significant as compared by the FMDV serotypes (P-Value < 0.05). The study result indicated that IgY antibody could be used for any immunological tests applied for FMD diagnosis. Moreover, the study finding indicated that chicken egg yolk antibody (IgY) production could be the best alternative to the other mammalian laboratory animals as egg yolk-based antibody production reduce the stress and bleeding during the blood collection, high antibody titer and low cost, easy to manage and handle chickens. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/25431 | |
| dc.language.iso | en | en_US |
| dc.subject | FMDV serotype A, O and SAT-2 | en_US |
| dc.subject | antibody, egg yolk IgY, serum | en_US |
| dc.subject | Percentage of inhibition and antibody titer | en_US |
| dc.title | Production of Foot-And-Mouth Disease (Serotype: O, A And Sat-2) Polyclonal Antibodies (Igy) By Using Layer Hens | en_US |
| dc.type | Thesis | en_US |