Aflatoxins (B1, B2, G1 And G2) and Aflatoxigenic Fungi In Export Standard White and Red Sesame Seeds (Sesamum Indicum L.) Grown In Humera and Wollega, Ethiopia
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Date
2017-06-03
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Addis Ababa University
Abstract
The oilseed sector is one of the fastest growing sectors in Ethiopia, both in terms of its foreign
exchange earnings and as source of income for millions of Ethiopians. Oilseed crops are
recognized to be potentially suitable substrates for the production of aflatoxins by aflatoxigenic
fungi. This study aimed to evaluate Knowledge attitude and practice (KAP) of sesame exporters
about aflatoxin contamination, analyze thousand seed weight, moisture, crude fat and peroxide
value of white and red sesame collected from Humera and Wollega varieties. Determining the
level of aflatoxins of white and red Ethiopian sesame seeds. Compare the level of aflatoxins
between white and red Humera and Wollega varieties. Isolate and identify aflatoxigenic fungi
from sesame seeds. A total of 27 sesame samples were collected from Ethiopian Agricultural
commodities warehouse service enterprise, located in Addis Ababa. Semi-structured
questionnaires used for knowledge, attitude and practice survey of sesame exporters and
Thousand seed weight, moisture, crude fat and peroxide value of sesame seeds have been done
according to American oil chemists society (AOCS) method. Analysis of aflatoxins was done by
using HPLC with solid phase extraction clean up method. Isolation and identification of
aflatoxigenic fungi has been done by culturing sesame samples on Potato dextrose agar (PDA)
media. Sixty percent of exporters doesn't have any idea about aflatoxins and all of them cannot
make aflatoxins test for sesame seed before export. Thousand seed weight, moisture, crude fat
and peroxide value were ranged 2.89-3.22g/1000 seed, 3.33-4.99 %, 46.75-57.75% and 2-8.2
meq/kg, respectively. Aflatoxins detected from 93 % of samples in the range of (0.44 - 48.28
ng/g). Average content of AFB1, AFB2, AFG1, AFG2 and total aflatoxins are 0.83 ng/g, 6.82
ng/g, 17.25 ng/g, 1.17 ng/g and 26.07 ng/g, respectively. AFB1 and AFG2 detected below
European union (EU) maximum limit for individual aflatoxin (2 ng/g). AFB2, AFG1 and total
aflatoxins content is above the permissible limit set by EU for both individual and total
aflatoxins (15 ng/g). Red sesame variety indicates relatively higher level of aflatoxins
contamination as compared with white Humera and white Wollega sesame. Aspergillus flavus, A.
parasiticus and A. niger isolates identified. Based on the finding, the contamination level may
affect national economy.
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Keywords
Sesame, Aflatoxins, HPLC, Solid Phase Extraction, Oilseed