EVALUATION OF THE IMMUNE RESPONSE OF NEWCASTLE DISEASE VIRUS VACCINES, IN LAYER CHICKENS
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Date
2018-06
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Abstract
This study was undertaken to evaluate the immune responses of NDV vaccines in layer chickens in the National Veterinary Institutes (NVI), Bishoftu, Ethiopia, in the period of February to May 2018.A total of 140 layer day old chicks (Lohmann Brown) were randomly divided into seven groups such as 1, 2, 3, 4, 5, 6, and 7 of which group 1, 2, and 3 were vaccinated primarily with HitchnerB1, LaSota, and Thermos-table I2 vaccine respectively at day 5 of age and secondarily vaccinated using LaSota, LaSota and Thermostable I2; vaccine, respectively at day of 26, again thirdly (second boost) vaccinated using Thermostable I2, Thermostable I2,Thermostable I2; vaccines,at the day of 54 of age, through single eyeinstillationof the same schedules and group 4,5 and 6 were vaccinated with the same vaccines respectively via double eye instillation following the same schedule.Group 7 was kept as unvaccinated control chickens.Sera samples were collected after 10 vaccination days and at day 5, 15, 21, 31 of age from unvaccinated control whereas at day of 15, 36, and 64 of age from vaccinated chickens.Serum sample subjected to Haemagglutination Inhibition (HI) test for the determination of antibody titres. It was observed that after secondary vaccination the geometric mean (GM) titres of vaccinated chickens were induced highest antibody titer than primary vaccinationand third vaccination GMtiter.From the present research it may be concluded that LaSota, LaSota, thermostable I2vaccination schedules was the best combination of which produced highest immune response in single eye instillation whereas HB1LaSota, LaSota, vaccination schedules was the best combination of which produced highest immune response in double eye instillation.The persistence of passive immunity remains in chickens until the age of day 31.
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A Thesis Submitted to the College of Veterinary Medicine and Agriculture of Addis Ababa University in partial fulfillment of the requirements for the degree of Master of Veterinary Science in Veterinary Microbiology
Keywords
Immune response, Layer chickens, Newcastle disease