Evaluation of Isolates of Pseudomonas Fluorescens As Biocontrol Agent Against Potato Bacterial Wilt Caused by Ralstonia (Pseudomonas) Solanacearum
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Date
2006
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Addis Ababa University
Abstract
A total of 50 bacterial isolates of fluorescent pseudomonas were isolated from
potato rhizosphere soil collected from different potato growing areas of the
country. The isolates were screened In Vitro for their antagonistic activity against
the pathogen, Ralstonia solanacearum on KB agar medium. Out of the 50
isolates, only three (Pfs2, Pfw1, Pfwt3) showed a certain degree of inhibition to
the growth of the pathogen. To test their antagonistic effect in a greenhouse, a
pot experiment was conducted using sterilized soil and potato tuber (CIP
383032.15) that was susceptible to the pathogen. In the experiment,
bacterization of potato tubers with the selected isolates i.e., Pfs 2, Pfw 1, Pfwt 3
and Pfri (reference strain) significantly reduced the incidence of bacterial wilt by
59.83%, and increased plant growth: plant height and dry weight by 76.89% and
28.44%, respectively suggesting the importance of the isolates as plant growth
promoting rhizobacteria.
Key words/Phrases: Potato, P. fluorescens, R. solanacearum, Antagonstic
Interaction
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Keywords
Potato, P. fluorescens, R. solanacearum, Antagonstic Interaction