Performance Evaluation of the First-Generation (1G) Color Plate Drug Susceptibility Testing Assay Identifying Drug- Resistant Mycobacterium Tuberculosis Isolates for Selected Anti- Tuberculosis Drugs
No Thumbnail Available
Date
2024-03-13
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababa University
Abstract
Tuberculosis is a major global public health concern and the emergence of drug- resistant (DR)-TB has been a major challenge to the control of the disease, especially in developing countries, including Ethiopia. Despite availability of appropriate diagnostic tools for Mycobacterium tuberculosis drug susceptibility testing, accessibility and timely diagnosis of DR-TB in resource-constrained settings, such as Ethiopia, is often a challenge. The first- generation color plate assay (1G) is ideally suited for low resource settings due to low cost, minimal technical expertise needed, and rapid provision of DST results.
Conclusion: The phenotypic DST 1G test is considered inexpensive (~$1.75), easy to use, rapid for identifying DR-TB using archived pulmonary and extra pulmonary MTB isolates from sputum and FNA and thus, it may be a viable option for DST in high endemic and resource limited settings like Ethiopia. The 1G test concurrently performs TB growth detection and DST in stored isolate’s, enabling timely treatment and preventing spread of DR-TB within the community. Additionally, the 1G test demonstrated good performance in detecting FNA (extra pulmonary) isolates, suggesting that it could be a useful alternative method for extra-pulmonary TB and DST diagnosis.
Key Words: 1G test, color plate test, indirect proportional DST Method, Multidrug-resistant tuberculosis, Pre-XDR Tuberculosis, Tuberculosis
Description
Keywords
1G test color plate test indirect proportional DST Method Multidrug-resistant tuberculosis Pre-XDR Tuberculosis Tuberculosis