Hepatoprotective Activity of Aqueous and Ethanol Extract of Lippia Adoensis Leaf Against Carbon Tetrachloride-Induced Hepatotoxicity in Mice
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Date
2015-03
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Addis Ababa University
Abstract
Medicinal plants may be the best source of remedies for the treatment of liver disease. Thus identification of a potential therapeutic agent for protection of liver from hepatotoxins provides a useful way for the prevention of liver related illnesses. The aim of the present study was to investigate the hepatoprotective activity of 80% ethanolic and aqueous extract of leaves of Lippia adoensis against carbontetrachloride in mice.
Mice were pretreated with extract of Lippia adoensis (200 & 400 mg/kg bw.po) for 7 days and then challenged with CCl4 (2ml/kg bw po.) on the 7th day. Serum biomarkers (AST, ALT, ALP and total bilirubin, total protein and albumin) were estimated in all the study groups. Alteration in the levels of biochemical parameters of hepatic damage like AST, ALT, ALP and total bilirubin, total protein and albumin were tested in both CCl4 and Lippia adoensis extract treated groups. CCl4 significantly (P<0.001) increased the AST, ALT, ALP and total bilirubin levels and decrease the level of total protein and albumin. Pretreatment with the extract of Lippia adoensis (200 & 400 mg/kg) produced significant (P<0.001) hepatoprotective effects as evidenced by decreased serum enzymes (AST, ALT, ALP) and serum bilirubin and increase the level of total protein and albumin as well as by histopathological findings of the liver. Histopathological examination of the liver tissues of CCl4 group represented the presence of marked foci of mononuclear infiltration in the hepatic parenchyma tissue, sinusoid and around central vein, as well as disorganization of hepatic plates, while the pretreatment with extract of Lippia adoensis overcome most of these changes to normal histological architecture of the liver in treated groups, compared to the controls. Change in the body weight of Lippia adoensis extract treated groups increased significantly (P < 0.001) as compared to carbontetrachloride treated group. The absolute and relative liver weights of extracts treated mice decreased significantly (P < 0.001) as compared to those treated with CCl4. Both ethanolic and aqueous extract of Lippia adoensis were comparable with the standard drug, Silymarin. The experimental results indicate that, ethanolic and aqueous extract of Lippia adoensis has potential hepatoprotective effect.
Key words: Lippia adoensis, Hepatoprotective, CCl4, Silymarin, AST, ALT, ALP, Bilirubin, Protein and Albumin, Ethanol extracts, Aqueous extract, Hepatotoxicity, mice.
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Keywords
Lippia adoensis, Hepatoprotective