Effect of Antibiotics in Eliminating Bacterial Wilt (Ralstonia Solanacearum) From in Vitro Propagated Ginger (Zingiber Officinale Roscoe)
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Date
2019-01-03
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Addis Ababa University
Abstract
Ginger (Zingiber officinale Rosc.) is a perennial herb. It belongs to the family Zingiberaceae and commercially cultivated in most tropical regions of the world. The underground rhizomes are the planting materials in conventional propagation of ginger. However, it is affected by diseases that cause tissue senescence and degeneration. Heavy losses of rhizomes have been observed because of infection with Ralstonia solanacearum. A disease-free planting material is necessary in order to get a successful ginger cultivation. Plant tissue culture technology has been successfully used for the commercial production of pathogen-free plants and for germplasm conservation of rare and endangered species. In vitro culture techniques provide an alternative means of plant propagation and a tool for crop improvement. Ginger Multiplication media are often contaminated with Ralstonia solanacearum the bacteria may survive endophytically within these plantlets thereby making the plantlets unusable for in vitro propagation. An experiment was conducted to find out effectiveness of antibiotics against the growth of Ralstonia solanacearum under in vitro condition. Four antibiotics namely, Gentamicin, Tetracycline, Ampicillin, Streptomycin with four concentrations, 130 mg/l, 160 mg/l, 200 mg/l and 250 mg/l were used to test their effectiveness in eliminating bacteria from in -vtro propagating ginger .Gentamicin was found to be superior to other treatments with highest inhibition (22 mm) at 250 mg/l followed by Tetracycline at 200 ,mg/l (18mm), Streptomycin 13 mm at 130mg/l and Ampicillin 12 mm at 130mg/l. Therefore, using antibiotics in ginger micro propagation medium can eliminate Ralstonia solanacearum from in vitro propagated ginger.
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Inhibition Zone, Invitro Susceptibility Test, Isolation and Identification