Evaluation of in vivo wound healing activity of 80% methanol crude extracts of the bulbs of Crinum abyssinicum in Mice
| dc.contributor.advisor | Pro.Makonnen, Eyasu( PhD) | |
| dc.contributor.advisor | Eguale, Tadesse(PhD) | |
| dc.contributor.author | Hussein, Abdi | |
| dc.date.accessioned | 2020-03-10T12:00:09Z | |
| dc.date.accessioned | 2023-11-06T08:08:11Z | |
| dc.date.available | 2020-03-10T12:00:09Z | |
| dc.date.available | 2023-11-06T08:08:11Z | |
| dc.date.issued | 2019-04 | |
| dc.description.abstract | Crinum abyssinicum is a species of bulbous plant that belongs to the family Amaryllidaceae. It is believed that the plant is native to Ethiopia, and known by the local name yejibb shinkurt (in Amharic). This plant is traditionally used for treatment of wound. No scientific evidence which substantiates its traditional claim is, however, available to date. The objective of this study is therefore to evaluate the wound healing activity of bulbs extracts of C. abyssinicum in mice. Mice were used for acute toxicity, wound healing and anti-inflammatory studies. For wound healing study, the 80% methanol extract of C. abyssinicum bulbs were formulated for topical application by incorporating it in simple ointment base B.P. in a concentration of 5% (w/w) and 10% (w/w). The simple ointment and nitrofurazone (0.2%) skin ointment were used as negative and positive controls, respectively. Toxicity of the formulated ointments was studied by skin irritation test on Swiss albino mice. For anti-inflammatory study, carrageenan-induced hind paw edema model was used to evaluate the anti-inflammatory effect of the extract at concentrations of 100, 200 and 400 mg/kg. Mice treated with Indomethacin (10mg/kg) and 2% Tween 80 served as positive and negative controls, respectively. The antimicrobial activity of the extract at concentrations of 50, 100 and 200 mg/ml was studied using agar well diffusion assay. Level of wound contraction, period of epithelialization, skin breaking strength, edema and zone of inhibition were determined. The ointment formulation of the extract was found to be non-irritant at 5% and 10% concentrations. Treatment of wound with ointment containing 5% (w/w) and 10% (w/w) of 80% methanol extract of the bulbs exhibited significant increase in wound contraction rate, shorter epithelialization time and higher skin breaking strength in the excision and incision wound model used as compared to control. The plant extract also showed dose-dependent significant reductions (p < 0.05) of inflammation compared to control. The wound healing potentials, anti-inflammatory and antimicrobial effect of the 80% methanol extracts of C. abyssinicum bulb observed in this study is consistent with the traditional claim of this plant for wound healing activity, and hence it is iii recommended to perform fractionation of crude extract in order to identify which fraction/s of the plant extract is/are responsible for wound healing. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/21095 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Abeba University | en_US |
| dc.subject | Crinum abyssinicum, wound healing, excision, incision, anti-inflammatory, antimicrobial | en_US |
| dc.title | Evaluation of in vivo wound healing activity of 80% methanol crude extracts of the bulbs of Crinum abyssinicum in Mice | en_US |
| dc.type | Thesis | en_US |