Genetic Diversity of an African Wild Rice (Oryza Longistaminata Chev. ET Roehr) From Amhara and Gambela Regions As Revealed by Microsatellite Markers
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Date
2011-11
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Addis Ababa University
Abstract
Data from microsatellite markers have been extensively used for both
in situ and ex situ conservation strategies through determining the
level of genetic diversity of natural populations that can widen the
gene pool of cultivated plants. Such conservation practices should also
lie on understanding the between and within population genetic
variation and partitioning populations on the basis of geographic
origin. Therefore, the objective of this study was to assess the intraspecific
diversity in Oryza longistaminata and how the variation is
partitioned within and between different geographic regions of the
eight Oryza longistaminata populations in Ethiopia using simple
sequence repeat markers. The five microsatellite markers in 320
samples generated 64 allels that revealed the presence of large amount
of genetic variability (Ho =0.225; He = 0.768; Na = 7.375; Ne = 6.565
and P = 0.744). The F-statistics detected by the microsatellite loci
showed Fst = 0.064 and Fis = 0.743 and there was no population in
Hardy-Weinberg equilibrium. The genetic parameters estimated from
this data indicated that there are high levels of genetic diversity in the
populations of O. longistaminata studied and this diversity is higher
within than between populations. Regional partitioning also revealed
that most of this diversity is found between populations within regions
than among regions. Among all the populations sampled, five
populations (EG-4 and all Amhara populations) were identified as
priorities for conservation strategies and thus, national collection and
conservation strategies need to consider population and regional
differences.
Key words or phrases: genetic variability, microsatellites, Molecular
markers, Oryza longistaminata, populations and simple sequence
repeats.
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Keywords
genetic variability, microsatellites,, Molecular markers, Oryza longistaminata, populations and simple sequence repeats.