Evaluation of the Anti-diarrheal Activity of 80% Methanol Extract and Solvent Fractions of the leaves of Lantana camara Linn (Verbenaceae) in Mice

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Addis Ababa University


Lantana camara L. is one of the medicinal plants traditionally used for the treatment of diarrhea in Ethiopia. Although the antidiarrheal activity of the crude extract of the leaves of this plant was evaluated elsewhere, no work has been performed in the Ethiopian plant. The aim of this study was therefore to further confirm the antidiarrheal activity of 80% methanol extract and fully evaluate the solvent fractions using mice model of diarrhea. The 80% methanol extract was prepared by maceration and the fractions were obtained by successive soxhlet extraction with chloroform and methanol followed by maceration of the marc with water. The antidiarrheal activity of the extract and fractions were investigated using castor oil induced diarrhea (for 80% methanol extract), enteropooling and small intestine transit models (the three models for the fractions). The test groups received various doses (100, 200 and 400 mg/kg) of the extract or fractions, whereas positive controls received Loperamide (3 mg/kg) and negative controls received vehicle (2% tween 80 or distilled water, 10 ml/kg). In the castor oil induced diarrheal model, the 80% methanol extract delayed onset of defecation, at 200 mg/kg and 400 mg/kg, and reduced the number and weight of feces at all tested doses significantly as compared to the negative control. In this model the methanol and aqueous fraction at all tested doses and chloroform fraction at 200 mg/kg and 400 mg/kg significantly reduced the number and weight of wet feces when compared with negative control. In the enteropooling test, the methanol and aqueous fractions significantly reduced the weight and volume of intestinal fluid at all tested iv doses, whereas the chloroform fraction significantly reduced the weight of intestinal content only at 400 mg/kg when compared to negative control. Results from the charcoal meal test revealed that all the fractions produced a significant anti-motility effect at all tested doses as compared to negative control. This study confirmed the antidiarrheal activity of the crude extract and further revealed all the three fractions possessed varying degree of antidiarrheal activity, with the methanol fraction being the most active fraction in all the three models. Key words: antidiarrheal activity, castor oil induced diarrhea, anti-enteropooling



Antidiarrheal activity; Castor oil induced diarrhea; Anti-enteropooling