Spectrophotometric Determination of Sulphate with BariumHematoxylin Complex
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Date
1994-06
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Addis Ababa Universty
Abstract
The determination of sulphate by using Barium -
hematoxylin complex has been done for the quantitative
determination of sulphate in soil and ground water samples.
The formation of complex between Barium and hematoxylin has
been studied and the absorbance was measured
spectrophotometrically at Amax 556nm. The ratio of the ligand
to metal was dormant by mole ratio method and was found to be
1:1. The reaction of a sample solution containing sulphate
with the Barium-hematoxylin complex decreases the absorbance
of the complex and this decrease in absorbance is directly
proportion at to the amount of sulphate present and this was
the basis of our method. The effect of experimental variables
has been studied to establish the optimum conditions of the
complex formation. It has been found that consistent and
reproducible results are obtained by carrying out the
reaction at room temperature for 30 minutes. The colour
system has been found to obey Beer's law in the concentration
range 0.5 - 2.2 ~g ml-1 of sulphate. The detection limit and
precision of the method were found to be 0.014 ~g/ml and
0.71% respec;: vely. Od thfJ basis of these studies, a simple and precise method has been developed for the determination
of sulphate. The method has been developed to the analysis
of soil samples and ground water. The results obtained have
been compared with other methods. The proposed method has
been found to give results which are in good agreement with
those of routinely used methods.
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Keywords
determination of sulphate