Spectrophotometric Determination of Sulphate with BariumHematoxylin Complex

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Addis Ababa Universty


The determination of sulphate by using Barium - hematoxylin complex has been done for the quantitative determination of sulphate in soil and ground water samples. The formation of complex between Barium and hematoxylin has been studied and the absorbance was measured spectrophotometrically at Amax 556nm. The ratio of the ligand to metal was dormant by mole ratio method and was found to be 1:1. The reaction of a sample solution containing sulphate with the Barium-hematoxylin complex decreases the absorbance of the complex and this decrease in absorbance is directly proportion at to the amount of sulphate present and this was the basis of our method. The effect of experimental variables has been studied to establish the optimum conditions of the complex formation. It has been found that consistent and reproducible results are obtained by carrying out the reaction at room temperature for 30 minutes. The colour system has been found to obey Beer's law in the concentration range 0.5 - 2.2 ~g ml-1 of sulphate. The detection limit and precision of the method were found to be 0.014 ~g/ml and 0.71% respec;: vely. Od thfJ basis of these studies, a simple and precise method has been developed for the determination of sulphate. The method has been developed to the analysis of soil samples and ground water. The results obtained have been compared with other methods. The proposed method has been found to give results which are in good agreement with those of routinely used methods.



determination of sulphate