Lignocellulosics for Cellulase and Fungal Biomass Production
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Date
1994-06
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Addis Ababa University
Abstract
The effects of teff straw (Eragrostis tett) or corn stalk
(Zea mays) on cellulase and biomass production by Trichoderma
sp. BDCC-1, Penicillium sp. BDCC-2 and Cladosporium sp. BDCC-3
were studied and compared with those of cellulosic substrates.
In Trichoderma sp. both teff straw and corn stalk were
found to be superior to filter paper for inducing B-GDase as
well as for improving fungal biomass production. CMCase and
FPase productions were, however, lower on these substrates than
they were on filter paper.
In Cladosporium sp. teff straw and corn stalk were found
to be better than CMC for inducing both CMCase and FPase.
However, no B-GDase activity was detected when only these
lignocellulosics were used in the media. It's biomass
production was also relatively lower on these substrates as
compared to that it produced on 1% CMC.
Penicillium sp. produced relatively large amounts of
biomass, CMCase, FPase and B-GDase on 2% corn stalk as compared
to those it produced on 2% CMC.
Lye- and 2% NaOH-pretreatments were generally effective in
improving biomass production in the test fungi. But their
effect on CMCase, FPase and B-GDase production was not similar
for the three fungi. There was, however, no significant
difference between Lye- and 2% NaOH-pretreatment in their
performance on biomass and cellulase production.
65-80% of the polysaccharides of alkali-treated teff straw
and corn stalk were hydrolysed to reducing sugars in 72 hours
using culture filtrate of Trichoderma sp. BDCC-1 which
consisted of 1.7 IU/ml of FPase. The hydrolysates were found to
support good growth of Candida utilis BDCC-25 (16.7-24.8mg dry
wt./100ml) and Saccharomyces cerevisiae BDCC-24 (14.6-15.6mg
dry wt./100ml). The yields of yeast biomass produced on these
hydrolysates were higher than those produced on D-glucose
(0.02%). It was, therefore, concluded that teff straw and corn
stalk could provide cheap substrates for mold biomass
production as well as for single-cell protein production if
they are pretreated with Lye.
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Biology