OUTBREAK INVESTIGATION, ISOLATION AND MOLECULAR CHARACTERIZATION OF SHEEP AND GOAT POX VIRUS IN CENTERAL ETHIOPIA
| dc.contributor.advisor | Dr.Addisu Demeke, Dr. Esayas Gelaye | |
| dc.contributor.author | Aberaham, Damena | |
| dc.date.accessioned | 2018-09-26T06:10:56Z | |
| dc.date.accessioned | 2023-11-30T12:54:16Z | |
| dc.date.available | 2018-09-26T06:10:56Z | |
| dc.date.available | 2023-11-30T12:54:16Z | |
| dc.date.issued | 2018-06 | |
| dc.description | A Thesis submitted to the College of Veterinary Medicine and Agriculture of Addis Ababa University in partial fulfillment of the requirements for the degree of Master of Science in Veterinary Microbiology | en_US |
| dc.description.abstract | A cross sectional observational study was conducted from September, 2017 to March, 2018 to outbreak investigation, isolation and molecular characterization of sheep and goat pox virus in different districts of central Ethiopia. Study was engaged different approaches especially questionnaire survey, virus isolation using vero cell line, classical PCR for amplification of the DNA, gel-electrophoresis for identification of the specific band, real time PCR for further genotyping, sequencing the RP030 gene for identification of genetic relationship of sheep and goat pox virus isolate from field with other Capri pox virus (CaPV).The questionnaire survey results indicated that sheep and goat pox was the most common disease in all the study areas and the disease was frequently seen. The disease outbreak more (52.94%) observed in rainy season. Additionally, non vaccinated animals, female and young age shoats were more affected (P< 0.05) by pox virus. A total of 712 sheep and goats (603 sheep and 109 goats) were clinically examined for the presence of pox lesions on their skin and 35.82% sheep and 28.44% goats had pox lesions. Generally, high mortality (9.95%) rate was observed in sheep than goats respectively. The virus was isolated from 16 skin samples (13 sheep and 3 goats) and the cell culture showed a typical characteristic of pox virus induced-cytopathic effect with destruction of monolayer and rounding of the cell. Similarly, the conventional PCR revealed that 16 out of 16 tested samples were positive by developing band size of 172bp (Goat pox virus). Further more real time PCR 16 tested samples were positive for goat pox virus with the melting temperature of (560C/72.50C). Phylogenetic tree analysis revealed that the RP030 gene (606 nucleotides) sequences of the present isolates originated from both sheep and goats were clustered with the goat pox virus group. Even though the existing information suggested that capri pox virus is strictly host specific, but in the current study the PCR and RP030 gene sequencing result confirmed that sheep were infected by goat pox virus similarly to goat pox virus and hence classification of pox virus based on infected host in small ruminant has been found to be inconclusive. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/12234 | |
| dc.language.iso | en | en_US |
| dc.subject | Central Ethiopia | en_US |
| dc.subject | outbreak investigation | en_US |
| dc.subject | sheep and goat pox viruses | en_US |
| dc.title | OUTBREAK INVESTIGATION, ISOLATION AND MOLECULAR CHARACTERIZATION OF SHEEP AND GOAT POX VIRUS IN CENTERAL ETHIOPIA | en_US |
| dc.type | Thesis | en_US |