Genetic Variability Based on Isozyme Analysis in Noug (Guizotia Abyssinica) Populations Collected From Gojam and Welo, Ethiopia
No Thumbnail Available
Date
2006-07
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababa University
Abstract
Isozyme electrophoresis was used to assess genetic variability in twelve
populations of Guizotia abyssinica collected from Gojam and Welo regions.
Three enzyme systems namely, phosphoglucose isomerase (PGI; E.C.5.3.1.9),
ß-esterase (ß-EST; E.C.3.1.1.-), and aspartate aminotransferase (AAT;
E.C.2.6.1.1.) were studied. A total of 17 alleles were observed from eight
genetic loci for the three enzyme systems from which five loci were
polymorphic. The mean percentage polymorphic loci (PP), the mean number of
alleles per locus (AP), the mean observed heterozygosity (Hop) and the mean
expected heterozygosity (Hep) showed the range 37.5-62.5%, 1.625-1.875,
0.1875-0.3375 and 0.1901-0.2599, respectively. The mean population
differentiation (FST) estimated was 0.2117. The averages of mean gene
diversities of Gojam and Welo populations pooled separately were 0.2307 and
0.2064, respectively. The genetic distance calculated for each pair wise
comparison ranged from 0.0012-0.2346. The results indicate the presence of
high genetic variability within and between populations of Guizotia abyssinica.
The results also show higher level of within population variability in Gojam
than Welo populations. Dendrogram drawn based genetic distance revealed
some level of association between populations of the same region.
Description
Keywords
Biology