Genetic Variability Based on Isozyme Analysis in Noug (Guizotia Abyssinica) Populations Collected From Gojam and Welo, Ethiopia

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Addis Ababa University


Isozyme electrophoresis was used to assess genetic variability in twelve populations of Guizotia abyssinica collected from Gojam and Welo regions. Three enzyme systems namely, phosphoglucose isomerase (PGI; E.C., ß-esterase (ß-EST; E.C.3.1.1.-), and aspartate aminotransferase (AAT; E.C. were studied. A total of 17 alleles were observed from eight genetic loci for the three enzyme systems from which five loci were polymorphic. The mean percentage polymorphic loci (PP), the mean number of alleles per locus (AP), the mean observed heterozygosity (Hop) and the mean expected heterozygosity (Hep) showed the range 37.5-62.5%, 1.625-1.875, 0.1875-0.3375 and 0.1901-0.2599, respectively. The mean population differentiation (FST) estimated was 0.2117. The averages of mean gene diversities of Gojam and Welo populations pooled separately were 0.2307 and 0.2064, respectively. The genetic distance calculated for each pair wise comparison ranged from 0.0012-0.2346. The results indicate the presence of high genetic variability within and between populations of Guizotia abyssinica. The results also show higher level of within population variability in Gojam than Welo populations. Dendrogram drawn based genetic distance revealed some level of association between populations of the same region.