The .in and Vivo Nitrate Reeducates Activity in Tef (Eragrostis Tef (Zucc.) Trotter) Under Different Assay Conditions
| dc.contributor.advisor | Zeleke, Asfaw ( PhD) | |
| dc.contributor.author | Negash, Legsse | |
| dc.date.accessioned | 2018-07-03T10:48:06Z | |
| dc.date.accessioned | 2023-11-08T16:32:44Z | |
| dc.date.available | 2018-07-03T10:48:06Z | |
| dc.date.available | 2023-11-08T16:32:44Z | |
| dc.date.issued | 1982-06 | |
| dc.description.abstract | The presence, substrate inducibility and regulations of the enzyme nitrate reductase (NADH: Nitrate Oxidoreductase, . . E.C. 1.6.6 .• 1)(NR) of both dark-grown and light-grown tef plants (Eragrostis ~ (Zucc.) 'rrotter) were studied. The relative merits of the in vivo and in vitro methods of assay for the enzyme were evaluated. Major factors affecting nitrate reductase activity and the distribution of the enzyme in both the dark- and light-grown plants were investigated. Nitrate and urea induced the formation of nitrate reductase in 5-day-old dark-grown tef plants; and the activity of the enzyme was predominantly associated with the roots. In the glasshouse-grown plants, however, both the ammonia-type and the nitrate-type nitrogen sources elicited NR activity although nitrate was still superior in enzyme induction; and the leaves were found to be the major centeres for nitrate reduction. A pH of 7.6 was found to be optimal for an in vivo NR activ~ty of tissue obtained from leaves of light-grown tef plants. Diurnal variations in NR activity were observed, with peak activity at mid-day, suggesting that the enzyme is under the control of light •. Logging tef plants for 11 days after 10 days of maturation did not substantially affect the enzyme activity. It was observed that tef seedlings discriminate between ammonium and nitrate during their early stages of development by preferentially assimilating the former when given in combination with the latter. These findings suggest that tef plants can, at least at their early stages of development, successfully withstand reductive soil conditions. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/5874 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Biology | en_US |
| dc.title | The .in and Vivo Nitrate Reeducates Activity in Tef (Eragrostis Tef (Zucc.) Trotter) Under Different Assay Conditions | en_US |
| dc.type | Thesis | en_US |