Extraction and Clean-up Techniques for Selective Isolation of Atrazine and Ametryn Residues from Soil Samples of Wonji Shoa Sugar Cane Plantation Farm
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Date
2007-07
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Addis Ababa Universty
Abstract
In this research work atrazine and ametryn, which are applied as mixture known by brand
name of i W to sugar cane plantation farms were extracted employing two conventional
extraction techniques, namely, solvent shake and soxhlet. The extraction was carried out
using soil samples collected from wonji shoa sugar cane plantation farm, following the
standard procedures. Prior to the analyte extraction, moisture contents of the soil samples
were determined. It has been found that for all samples the moisture content values range
between 4.6-7.7 %. Thus, the soil samples were over dried and extraction of the target
compounds was performed. Moreover, since the colour of the resulting extracts was light
yellow and appeared to be turbid, further cleaning has been arranged. Clean-up of all
extracts were therefore utilizing florisil packed in a glass column and topped with
anhydrous sodium sulphate. The eluate from the glass column, which was nearly
colourless, and assumed to be free of particulate matters was evaporated to dryness and
reconstituted in 1.5 ml acetonitrile. It was then stored in a refrigerator, at 4 oC until used
for final analysis by HPLC.
Wonji Shoa sugar industry is one of the biggest sugar industries in Ethiopia. A mixture of
atrazine and ametryn (Gesapax Comb 500FW) is frequently applied herbicides in wonji
shoa sugar cane plantation. Soxhlet extraction and solvent shake extraction methodology
have been applied for extracting atrazine and ametryn from the collected soil samples.
Five soil samples were collected from different fields for selective isolation of atrazine
and ametryn.
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Keywords
Atrazine and Ametryn