Extraction and Clean-up Techniques for Selective Isolation of Atrazine and Ametryn Residues from Soil Samples of Wonji Shoa Sugar Cane Plantation Farm

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Addis Ababa Universty


In this research work atrazine and ametryn, which are applied as mixture known by brand name of i W to sugar cane plantation farms were extracted employing two conventional extraction techniques, namely, solvent shake and soxhlet. The extraction was carried out using soil samples collected from wonji shoa sugar cane plantation farm, following the standard procedures. Prior to the analyte extraction, moisture contents of the soil samples were determined. It has been found that for all samples the moisture content values range between 4.6-7.7 %. Thus, the soil samples were over dried and extraction of the target compounds was performed. Moreover, since the colour of the resulting extracts was light yellow and appeared to be turbid, further cleaning has been arranged. Clean-up of all extracts were therefore utilizing florisil packed in a glass column and topped with anhydrous sodium sulphate. The eluate from the glass column, which was nearly colourless, and assumed to be free of particulate matters was evaporated to dryness and reconstituted in 1.5 ml acetonitrile. It was then stored in a refrigerator, at 4 oC until used for final analysis by HPLC. Wonji Shoa sugar industry is one of the biggest sugar industries in Ethiopia. A mixture of atrazine and ametryn (Gesapax Comb 500FW) is frequently applied herbicides in wonji shoa sugar cane plantation. Soxhlet extraction and solvent shake extraction methodology have been applied for extracting atrazine and ametryn from the collected soil samples. Five soil samples were collected from different fields for selective isolation of atrazine and ametryn.



Atrazine and Ametryn