Foot and Mouth Disease Outbreak Investigation in Selected Areas of Central and Southern Parts of Ethiopia
| dc.contributor.advisor | Eyob Hirpa | |
| dc.contributor.author | Tekalegn Desta: | |
| dc.date.accessioned | 2025-09-03T11:51:29Z | |
| dc.date.available | 2025-09-03T11:51:29Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Foot and mouth disease is a highly contagious Trans boundary disease causing significant economic losses in cattle in Ethiopia that caused by Virus belonging to the family Picornaviridae's genus Aphthovirus. A cross sectional study and outbreak investigation was performed from October 2023 to May 2025. The study included selected areas five regions of Ethiopia. The study objectives were to estimate the seroprevalence of FMD, identifying circulating serotypes in the study areas. The seroprevalence was determined by 3ABC-ELISA and the circulating serotype obtained from antibody was identified by SPC-ELISA. In active Outbreak area the virus was cultivated on BHK 21 Cell line and virus genome detected using RTPCR The circulating serotype identified by antigen captured ELISA (IZSLER, Brescia, Italy). Semi-structured questionnaire survey was used to assess the community’s knowledge’s, attitude and practice toward FMD. The overall seroprevalence from 314 cattle was 80.89% (254/314). The serotyping result from 22 strong seropositive selected samples showed concurrently 4 serotypes circulating in south Omo namely, the SAT2 (18/22), O (16/22), SAT1 (13/22) and A (10/22). There was statistically significant p-value difference in seroprevalence in South Omo Zone districts. Totally 28 sample were cultivated on BHK21 cell lines took from 54 animals of outbreak investigation area for virus isolation was shown 64 %( 18/28) samples of FMDV CPE positive and whereas, 28 sample pooled into 22 sample by locality for FMDV genome detection by RT-PCR. The molecular detection confirms 45% positive for FMDV. For serotyping 18 samples positives for FMDV CPE were used and two circulating serotypes were identified namely, serotype O, 66.7% (12/18), and 16.7% (3/18) of serotypes A, and mixed serotypes O and A, 16.7% (3/18). Knowledge, Attitude and Practices survey analyses showed the 95% respondent do not vaccinate their animal for FMD and do not quarantine diseased animal. In conclusion FMD is very important economical diseases in the study area therefore, awareness creation and Vaccination campaign to protect the Cattle against FMD is recommended | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/7327 | |
| dc.language.iso | en_US | |
| dc.publisher | Addis Abeba University | |
| dc.subject | Foot and Mouth disease | |
| dc.subject | Isolation | |
| dc.subject | Knowledge | |
| dc.subject | Attitude and Practices | |
| dc.subject | Molecular Detection Outbreak Investigation | |
| dc.subject | Sero-prevalence | |
| dc.title | Foot and Mouth Disease Outbreak Investigation in Selected Areas of Central and Southern Parts of Ethiopia | |
| dc.type | Thesis |