Investigation of Chemical Constituents and Anti- Oxidant Activities of the Essential Oils of Lippia Adoensis and Ocimum Sanctum L.
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Date
2020-07-24
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Addis Ababa University
Abstract
In this study essential oils of Lippia adoensis leaf, Ocimum sanctum L. leaf and stem (together without separation) and the mixture of the two plants from Bishoftu and Debre Berhan sample area was analyzed using Gas chromatography mass spectrometry (GC-MS) and its antioxidant activities were assessed using UV-Vis spectroscopy. Ocimum sanctum L. from Bishoftu sample area showed 12 compounds. The detected major compounds were -bisabolene (31.38%), 4- [(1E)-1,5-dimethyl-1,4-hexadien-1-yl]-1-methyl-Cyclohexen (25.56%), eucalyptol (17.12%). The essential oil from stem and leaf of Ocimum sanctum L. from Debre Berhan sample area showed 20 compounds. The major compounds were -bisabolene (24.45%), 4-[(1E)-1,5- dimethyl-1,4-hexadien-1-yl]-1-methylcyclohexen (19.61%) and eucalypto (13.42%). Lippia adoensis from Bishoftu sample area showed 15 compounds with major components as linalool (66.60%) and caryophyllene (4.28%) and Lippia adoensis from Debre Berhan sample area showed 12 compounds with major component as linalool (86.11%). The essential oil of the mixture of Lippia adoensis and Ocimum sanctum L. from Bishoftu sample area showed 18 compounds with major components as linalool (62.54%) and from Debre Berhan sample area showed 21 compounds major components as linalool (48.47%) and octadecane (5.47%). The antioxidant activities were assessed using UV-Visible spectroscopy. The radical scavenging activity of the essential oils of Lippia adoensis and Ocimum sanctum L. was assessed using DPPH. The antioxidant activities of the sample were determined by comparing DPPH inhibition and standard ascorbic acid DPPH inhibition. The measured average antioxidant activities for essential oil of Ocimum sanctum L. from Bishoftu and Debre Berhan exhibited DPPH radical scavenging activities of 96.48% and 96.17% at 100μg/mL, respectively. Lippia adoensis from Bishoftu and Debre Berhan sample area exhibited DPPH radical scavenging activities of 92.58% and 93.37% at 100 μg/mL, respectively and the essential oil of mixture of two plants (Lippia adoensis and ocimum sanctum) from Bishoftu and Debre Berhan sample area exhibited DPPH radical scavenging activities of 95.25% and 96.42% at 100μg/mL, respectively, which is comparable to that of ascorbic acid standard that exhibited a 98.08 % at the same concentration.
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Essential Oils, Antioxidant Activity, DPPH Assay Ocimum Sanctum L. and Lippia Adoensis