The Effect of Some Physico-chemical Factors on the Production and Activity of Cellulase and B-Glucosidase from Cladosporium SP . BDCC-3
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Date
1990-06
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Addis Ababa,University
Abstract
Conditions for the produC"tion, rele8se ... nd ... ctivi. y ot
cellulases and B-Glucosidase tro", (11'1"os orium sp. »OCC:-J were
investig8 ed. Comparisons !IIade- betwPt"n different carbon !'Iource-s lind
their concenTrations for maxi1!lum prorlu~tjon of cellulas8s and aGlucosidase
depicted 1 CHC 0.5' Avicel. 1.5'd.-c-I'11ulose. 0.5'
filter paper .. nd 0 . 5 cotton to be op timum. From the nitrogen
sources used . potassium nitr ate was found to be the nost utilizable
by Clados orium 5p. BDCC-J. The OPti~um conce ntrations of nitrate
for maximum production of the enzy~p.s were 1'. 1%. 2%. 2% and 1%
i n CHC. Avical . -.. -cellulose . filter paper and cotton containing
media. r espectively. The most appropri ate de terQent for the release
of cellulases and B- Glucosidase was O.J Tween-SO . The enzymes had
a wide range of pH . However . t hey were most active at pH 5.0 and
60°C. All enzymes l os t activity when i n cubate~ at 700C for 2 hours.
Thus five different optimized media were f ormulated. These were:-
1. Optimized CMe medium 2 (OCI'1-2) containing 1" CHC. 1% KNO) and
0.1% Tween - 80 plus the basal medium
2 . Optimized Av icel medium 2(0 ~M -2) conta ining 0 .5" Avicel. 1% KNOJ
and 0.1" Tween - 80 plus the basal medium.
J . Optimized .. -Cellulose medium '-(0':' H-2 ) containing 1 . 5%
~ -Cellulose. 2% KNO) and 0.1% Tween - 80 plus the basal
medium .
4 . Optimized filter paper medium 2(OFM-2) containinq 0.5% FP . 2
KNO) and 0.1 Tween - 80 plu~ the baSAl medium .
5. Optimized cotton medium 2(OCOH - 2) con t a ining 0.5 cotton. 1%
KNOJ and 0.1% Tween - SO plus thp basal medium .
The fungus produced maximum level o f CMC - al'le . Avicelase and
~~ -cell usl ese after 6 days o f incubAti on while Fp- asp, co t tonase
and .a-Glucosidase were produced J to 6 d~ys later. Cl adosporium sp .
BDCC- J produced Cx (cmc-8s e ) much hi.ghpr than CI ( ~v ice'8 s e . ~-'7 -
cellulase. FP-ase . cottonase) and R-Glucosidase. A comparative
study of free . cytoplasmic and membrane bound enzymes showed that
cellulases were extracellular while a-Glucosidase was CytoplAsmic .
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Keywords
the Eff ec t of Some Phys i co -C hemical Facto r s on the Production and Activity