Electrochemical Determination of Folic Acid at Polybromocresol Purple Modifed Glassy Carbon Elelctrode
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Date
2020-02-02
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Addis Ababa University
Abstract
Polybromocresolpurple (PBCP) was used for the modification of glassy carbon electrode
(GCE) to determine the electrochemical behavior of folic acid (FA) in 0.1 M PBS at pH 7.0
at the scan rate of 100 mV/s. The modified electrode was characterized and used to
electrochemically determine FA using cyclic and differential pulse voltammetry. The
oxidation peak current of FA at the modified electrode increased compared to the bare
GCE, hence indicating that the BCP displays a significant improvement on the
electrochemical oxidation of FA. The oxidation peak current of FA is proportional to its
concentrations in the range of 20-100 μM with 0.987 correlation coefficient. The detection
limit was found to be 1.772 μM. Based on the relationship between the oxidation peak
current and square root of scan rate, the overall electrode process was found to be diffusion
controlled at BCP/GCE. The modified electrode exhibited good sensitivity, and
reproducibility for the determination of FA, indicating the capable applications of the
modified electrode in real sample analysis.
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Keywords
Folic Acid, Modified Electrode, Polybromocresolpurple (Bcp), Electropolymerization, Cyclic Voltammetry, Differential Pulse Voltammetry