ISOLATION AND SEROPREVALENCE OF BRUCELLA: FROM DAIRY CATTLE IN AND AROUND ASELLA AND BISHOFTU TOWNS, ETHIOPIA
| dc.contributor.advisor | Dr.Gezahegne Mamo, Dr. Gobena Ameni | |
| dc.contributor.advisor | Prof. Tesfu Kassa | |
| dc.contributor.author | Minda, Asfaw | |
| dc.date.accessioned | 2020-06-18T08:42:30Z | |
| dc.date.accessioned | 2023-11-30T12:54:19Z | |
| dc.date.available | 2020-06-18T08:42:30Z | |
| dc.date.available | 2023-11-30T12:54:19Z | |
| dc.date.issued | 2014-06 | |
| dc.description.abstract | Brucellosis is considered by the Food and Agriculture Organisation (FAO), the World Health Organisation (WHO) and the Office International des Epizooties (OIE) as one of the most widespread zoonoses in the world. A cross-sectional study was conducted in Asella and Bishoftu towns of Oromia Region of Ethiopia to isolate and determine seroprevalence of dairy cattle brucellosis. A total of 570 dairy cattle from 35 herds were purposively included in the study based on abortion history. Out of 46 clinical sample cultured 6.52% (3/46) overall rate of isolation was found in the study area and all the three isolates were B. abortus based on biochemical test result. B. abortus was isolated from placental cotyledon 1/9 (11.1%), vaginal swab 2/23 (8.69%) while no isolate was obtained from milk and fetal abomasal content of dairy cattle. All serum samples collected were tested and confirmed serologically using card test, rose Bengal plate test (RBPT), Indirect enzyme linked immuno sorbent assay (iELISA) and Complement fixation test (CFT). Risk factors analysis was also conducted using Chi-square and multivariate logistic regression analysis. An overall seroprevalence was estimated 1.4% (95% CI: 0.241, 3.461) by CFT while 16 (2.81%), 15(2.63%) and 13 (2.28%) were found positive by card test, iELISA and RBPT, respectively. The higher seroprevalence of 3.23% was observed in and around Asella compared to Bishoftu (0.52%) town. A Chi-square computed statistical analysis indicated that origin (χ2=6.63; P<0.05), breed type (χ2= 8.49; P<0.05), abortion history (χ2=92.43; P<0.001) and abortion period (χ2=192.97; P<0.001) were the major risk factors for Brucella infection. In addition, adult age (1.82%), intensive management system (1.82%), large herd size (2.17%) and pluriparous (2.41%) cows were found with higher sero positivity of Brucella. Multivariable logistic regression statistical analysis revealed that origin and breed type were significantly associated with seropositivity (P<0.05). Conse-quently, origin was statistically identified to be the major risk factor for brucellosis to occur in relation to other factors (OR=7.56) while breed type (OR=0.19) was the second major risk factor. Therefore, more proactive measures should be taken to protect the cattle populations from Brucella infection to reduce its ecomonic impact to the dairy industry and the risk of zoonotic infection in exposed human population. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/21733 | |
| dc.language.iso | en | en_US |
| dc.subject | Asella | en_US |
| dc.subject | Bishoftu | en_US |
| dc.subject | Brucellosis | en_US |
| dc.subject | Dairy Cattle | en_US |
| dc.title | ISOLATION AND SEROPREVALENCE OF BRUCELLA: FROM DAIRY CATTLE IN AND AROUND ASELLA AND BISHOFTU TOWNS, ETHIOPIA | en_US |
| dc.type | Thesis | en_US |