Investigation of Genetic Diversity in Ethiopian Collections of Safflower (Carthamus tinctorius) using ISSR Markers

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Addis Ababa University


Safflower, Carthamus tinctorius, L. is an oilseed crop that belongs to the family Asteraceae, a diverse group of flowering plants that grow in many parts of the world. So far, the characterization of safflower using molecular markers has been limited. The objective of this study was to investigate the genetic diversity of safflower accessions collected from different regions of Ethiopia using ISSR molecular markers. For this purpose, seeds of seventy landrace accessions collected from four administrative regions of Ethiopia (Amhara, Oromia, Tigray and SNNPR) were obtained from the IBC and grown in greenhouse at Addis Ababa University, Faculty of Life Science. DNA was extracted from a bulk leaf sample of five randomly selected plants per accession using a triple CTAB extraction technique. Four primers were selected. The four selected ISSR primers produced a total of 43 bands across the 70 safflower accession. The number of amplified fragments with ISSR primers ranged from 6 to 14 per primer with varied in size of 100 to 1000 base pairs. Out of the 43 bands detected, 87.5% were polymorphic. In terms of region, Oromia showed the highest percentage of polymorphism (86.1%) with Tigray having the least polymorphism (20.9%). Safflower population from Oromia has 0.32 and 0.48 gene diversity and Shannon diversity index, respectively whereas Amhara population of safflower has shown 0.27 and 0.39 gene diversity and Shannon diversity index, respectively. AMOVA also showed that 98.9% of the variation is attributed to within population while 1.1% is among populations variation. Both UPGMA and neighbor joining trees based on Jaccard’s similarity coefficient showed weak grouping among individuals collected from the same regions. The results reveal the presence of higher genetic diversity that deserves conservation attention and sustainable use strategy to improve the productivity of safflower. Key words: - Carthamus tinctorius L, genetic diversity, ISSR molecular marker, safflower, polymorphism, Ethiopia.



Carthamus tinctorius L, genetic diversity, ISSR molecular marker, Safflower, polymorphism, Ethiopia