Polarographic Investigation Op B-N-Oxalyl-L-A,B-Diaminofr.Ofrionic Acid, A Neurotoxin Prom The Seeds Op Lathyrus Sativus(Guaya)
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Date
1969-06
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Addis Ababa Universty
Abstract
Polarographic investigations of £-N-Oxalyl-L-a > 0-Diaminopropionic
acid, ODAP, a neurotoxin from the seeds of Lathyrus
sativus(Guaya) have been made.
Aqueous solutions of ODAP in KC1 supporting electrolyte
gave a single polarographic wave with a half-wave potential of
-1 ,518V, This wave was found to be due to the irreversible
discharge of hydrogen. The reduction wave disappeared on
standing and may be due to intramolecular rearrangement leading
to lactone formation.
At more positive potential a second peak appears for pH’s
less than 2. The reduction wave shifts towards more positive
values as the pH decreases. This reduction wave could be due to
a direct reduction of the protonated amino acid,
Polarograms of qqueous solution of Cu(ll), Ni(ll ), Zn(ll),
Cd(ll), Pb(ll) and Co(ll) in KC1 supporting electrolyte were not
affected by the addition of ODAP, However, in ammonia buffer
( pH = 8,2) and when ODAP was added only Ni(ll) and Co(ll) showed
larger shifts in the differential pulse polarographic peak
potentials after one day,
Polarographic behavior of complexes of ODAP with Ni(ll) and
Co(ll) in borax buffer (pH = 9.2) have been studied. The reduction
of both complexes were polarographically irreversible.
The stability constant and ligand number of the Ni(ll)~ ODAP
complex in borax ( pH = 9 ,2) were determined. The simple and
complex metal ions were reduced irreversibly. The ligand number
was determined to be 2 and the stability constant calculated to
be log $= 7.4
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Keywords
B-N-Oxalyl-L-A,B-Diaminofr.Ofrionic Acid