ISOLATION AND MOLECULAR CHARACTERIZATION OF LUMPY SKIN DISEASE VIRUS IN CENTRAL ETHIOPIA
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Date
2018-06
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Abstract
Lumpy skin disease (LSD) is an infectious disease of cattle, caused by a Lumpy Skin Disease Virus. LSD Causes considerable economic losses due to emaciation, damage to hides, infertility and, loss of milk production. In Ethiopia the disease is distributed almost all regions and is regarded as one of the most economically important livestock diseases in the country. Outbreak investigations have been carried out at different regions of the country on different times. The current study focused on the identification of LSDV based on outbreak reports central Ethiopia. Outbreak survey found 8.77% morbidity, 2.12% mortality and 25.61% case fatality rates in the region. Skin lesion samples were collected from clinically sick cattle and virus isolated on cell culture and shown the characteristics CPE of the virus. The virus DNA was identified by amplifying the 172bp DNA fragment using real time and conventional PCR. Phylogenetic analysis of the RPO30 gene sequence revealed that, the present isolates are form matching with previously identified isolates from Ethiopia and also with the KS-1 vaccine strains. The present study also attempt to isolate virus on Vero, ESH-L and LT primary cell cultures and comparing their susceptibility. The study found that lamb testes primary cell was best suited for primary isolation of LSDV. Vero cell however was found to be less sensitive for LSDV primary isolation but isolation can be achieved through continuous passage of the virus.
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MSc Thesis
Keywords
Cattle, Cell Culture, LSD, LSDV