Effect of Growth Regulators Concentrations on in Vitro Propagation of Three Elite Sugarcane (Saccharum Officinarum L.) Cultivars Using Shoot Tip Culture
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Date
2017-03-05
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Addis Ababa University
Abstract
Conventional vegetative propagation of sugarcane generally has low multiplication rate
and allows distribution of diseases. This results in shortage of quality planting materials.
Micropropagation is currently the only practical means of achieving rapid, large-scale
production of disease-free quality planting material. The present study was conducted
with the objective of studying the in vitro propagation of elite sugarcane genotypes
(Q200,Q217 and Co-0238) using shoot tip explants. Experiments on shoot tip culture initiation,
shoot multiplication and in vitro rooting were laid out in completely randomized
design with 2x3x3, 4x5x3 and 3x5x3 factorial treatment arrangements respectively. Data
was subjected to analysis of variance (ANOVA) and significant means were separated
using Duncan's multiple range tests. With regard to shoot multiplication, genotype Q200
showed a maximum of 13.59 shoots per explant with 5.83cm shoot length on a medium
fortified with 2 mg/l BAP alone, while genotype Q217 produced a maximum of 15.28
shoots per explant with 5.37cm mean shoot length on a medium supplied with 2.0 mg/l
BAP and 0.25 mg/l kinetin. Likewise, Co-0238 produced maximum of 13.56 shoots per
explant with mean shoot length 6.50 cm on medium fortified with 1.5 mg/l BAP + 0.5
mg/l kinetin. Half MS semi solid medium containing 3.0 mg/l NAA induced the highest
rooting with mean root number per shoot of 14.93 for Q200. For Co-0238, half MS medium
supplemented with 5.0 mg/l NAA induced the highest rooting response with mean
root number per shoot of 18.40. Similarly, ½ MS medium supplemented with 1.5 mg/l IBA
in combination with 3.0 mg/l NAA induced highest rooting response with 16.50 mean
root number per shoot. In conclusion, this study can be used for rapid in vitro propagation
of these three elite sugarcane genotypes.
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Keywords
Genotypes, 6-Benzylaminopurine, Naphthalene Acetic Acid, Indole Butyric Acid, Kinetin